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Series GSE8307 Query DataSets for GSE8307
Status Public on Aug 08, 2008
Title Expression and neurobehavioral analyses in prosaposin deficient mice: Molecular alterations precede neuronal deficits
Organism Mus musculus
Experiment type Expression profiling by array
Summary Prosaposin encodes, in tandem, four small acidic activator proteins (saposins) with specificities for glycosphingolipids hydrolases in lysosomes. To explore the molecular mechanism(s) of disease progression, temporal transcriptome microarray analyses of cerebrum and cerebellum tissues were conducted using mRNA from three prosaposin deficiency mouse models: PS-NA (hypomorphic prosaposin deficiency), PS-/- (prosaposin null) and 4L/PS-NA (a V394L/V394L glucocerebrosidase mutation and PS-NA) mice. Our results indicate that regionally specific gene expression abnormalities preceded the histological and behavioral changes and CEBPD is a candidate regulator of brain disease in prosaposin deficiency. The alterations of gene expression are detected at birth and are more profound in cerebellum than cerebrum.
Keywords: disease-state analysis
Overall design In order to increase the temporal resolution of expression profile in brain, the disease progression in those models were inverstigated in two regions of brains (cerebellum and cerebrum) at three or four time points according to the genotypes. PS-/-: new born (0d), 10 days (10d), 20 days (20d), 25 days (25d); PS-NA: new born, 4 weeks (4w), 12 weeks (12w), 18 weeks (18w); 4L/PS-NA: 4 weeks (4w), 12 weeks (12w), 18 weeks (18w). The data from those models were analyzed relative to the corresponding wild type at same time point (0d, 10d, 20d, 4w, 12w, 18w).
Citation(s) 18673548
Submission date Aug 08, 2007
Last update date Feb 11, 2019
Contact name nupur dasgupta
Phone 513-803-1768
Organization name Cincinnati Children's Hopital Medical Center
Street address 3333 Burnet Ave.
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (68)
GSM205934 cerebellum_PS-/-_ 0d_rep1
GSM205935 cerebellum_PS-/-_ 0d_rep2
GSM205936 cerebellum_PS-/-_ 10d_rep1
BioProject PRJNA101277

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Supplementary file Size Download File type/resource
GSE8307_RAW.tar 235.4 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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