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Series GSE8310 Query DataSets for GSE8310
Status Public on Jun 27, 2007
Title Gene expression data from hypothalamus of normal sexual development of the female rat
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary To determine the global changes in hypothalamic gene expression that may occur at the time of female puberty in this species, animals were euthanized at three different stages, juvenile (25-days of age), early puberty (30-35 days of age) and on the day of the first proestrus (32-37 days of age). According to criteria previously established (Ojeda,S.R.; Urbanski,H.F. Puberty in the rat pp.363-409. The Physiology of Reproduction, 2nd Edition, Vol 2. Edited by Knobil,E.; Neill,J.D. Raven Press, 1994), 25-day-old animals are in the mid- juvenile phase of prepubertal development (JUV). At this time, the vagina is not yet patent and the uterine weight is 60 mg or less, with no accumulation of intrauterine fluid. Older rats showing a closed vagina, accumulation of intrauterine fluid and an uterine weight less that 180 mg are considered to be in the early proestrous (EP) phase of puberty. Finally, rats still exhibiting a closed vagina, but showing a uterus ballooned with fluid and a uterine weight of at least 200 mg are considered to be in late proestrus (LP), i.e., the phase of puberty when the first preovulatory surge of LHRH and gonadotropins takes place. The first ovulation occurs the following day. All animals were sacrificed between 1600-1700 h, and the medial basal hypothalamus (MBH) was immediately dissected, as previously described (Rogers,L.C.; Junier,M-P.; Farmer,S.R.; Ojeda,S.R. A sex-related difference in the developmental expression of class II -tubulin messenger RNA in rat hypothalamus. Mol.Cell.Neurosci., 2: 130-138, 1991) and stored in RNAlater according to manufacturer’s instructions.
Keywords: comparison between Early pubertal and Juvenile or Late pubertal and Juvenile stages of sexual development in the female rat
Overall design Affymetrix arrays were used to detect global changes in gene expression in the hypothalamus of normal sexual development of the female rat.
Contributor(s) Lomniczi A, Mastronardi CA, Ojeda S
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Submission date Jun 27, 2007
Last update date Jul 31, 2017
Contact name Alejandro Lomniczi
Phone 503-690-5305
Fax 503-690-5384
Organization name Oregon National Primate Research Center - OHSU
Department Neuroscience
Street address 505 NW 185th Ave
City Beaverton
State/province OR
ZIP/Postal code 97006
Country USA
Platforms (1)
GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array
Samples (9)
GSM206008 hypothalamus of juvenile (JUV) rat_JUV1
GSM206009 hypothalamus of juvenile (JUV) rat_JUV2
GSM206010 hypothalamus of juvenile (JUV) rat_JUV3
BioProject PRJNA101283

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8310_RAW.tar 27.7 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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