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| Status |
Public on Mar 27, 2008 |
| Title |
A LysM Receptor-like Kinase Mediates Chitin Perception and Fungal Resistance in Arabidopsis |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
A LysM Receptor-like Kinase Mediates Chitin Perception and Fungal Resistance in Arabidopsis
Jinrong Wan,1 Xuecheng Zhang,1 David Neece,2 Katrina M. Ramonell,3 Steve Clough,2,4 Sung-yong Kim,1 Minviluz Stacey,1 and Gary Stacey1*
1Division of Plant Sciences, National Center for Soybean Biotechnology, C.S. Bond Life Sciences Center, University of Missouri-Columbia, Columbia, MO 65211, USA
2Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
3Department of Biological Sciences, University of Alabama, Tuscaloosa, AL 35487, USA
4US Department of Agriculture, Soybean/Maize Germplasm, Pathology and Genetics Research, Urbana, IL 61801, USA
*To whom correspondence should be addressed. E-mail: staceyg@missouri.edu
Abstract: Chitin, a polymer of N-acetyl-D-glucosamine, is found in fungal cell walls, but not in plants. Plant cells are capable of perceiving chitin fragments (chitooligosaccharides) to trigger various defense responses. We identified a LysM receptor-like protein (AtLysM RLK1) that is required for the perception of chitooligosaccharides in Arabidopsis. Mutation of this gene blocked the induction of almost all chitooligosaccharide-responsive genes (CRGs) and led to more susceptibility to fungal pathogens, but not to a bacterial pathogen. In addition, exogenously applied chitooligosaccharides enhanced resistance against both fungal and bacterial pathogens in the wild-type plants, but not in the mutant. Together, our data strongly suggest AtLysM RLK1 is the chitin receptor or a key part of the receptor complex and chitin is a PAMP (pathogen-associated molecular pattern) in fungi recognized by the receptor leading to the induction of plant innate immunity against fungal pathogens. Since LysM RLKs were also recently shown to be critical for the perception of the rhizobial lipo-chitin Nod signals, our data suggest that LysM RLKs not just recognize friendly symbiotic rhizobia (via their lipo-chitin Nod signals), but also hostile fungal pathogens (via their cell wall chitin). These data suggest a possible evolutionary relationship between the perception mechanisms of Nod signals and chitin by plants.
Keywords: chitooctaose, chitin receptor mutant
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| Overall design |
wild type Col-0 and chitin receptor mutants treated with or without chitooctaose
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| Contributor(s) |
Wan J, Clough S, Stacey G |
| Citation(s) |
18263776 |
| Submission date |
Jun 28, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Gary Stacey |
| E-mail(s) |
staceyg@missouri.edu
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| Phone |
573-884-4799
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| URL |
http://psu.missouri.edu/staceylab/index.htm
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| Organization name |
University of Missouri-Columbia
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| Department |
Plant Sciences
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| Street address |
1201 Rollins Road
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| City |
Columbia |
| State/province |
MO |
| ZIP/Postal code |
65211 |
| Country |
USA |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (12)
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| GSM206274 |
Wild type, chitooctaose treatment, biological rep1 |
| GSM206275 |
Wild type, chitooctaose treatment, biological rep2 |
| GSM206276 |
Wild type, chitooctaose treatment, biological rep3 |
| GSM206277 |
Wild type, water treatment, biological rep1 |
| GSM206278 |
Wild type, water treatment, biological rep2 |
| GSM206279 |
Wild type, water treatment, biological rep3 |
| GSM206280 |
Chitin receptor mutant, chitooctaose treatment, biological rep1 |
| GSM206281 |
Chitin receptor mutant, chitooctaose treatment, biological rep2 |
| GSM206282 |
Chitin receptor mutant, chitooctaose treatment, biological rep3 |
| GSM206283 |
Chitin receptor mutant, water treatment, biological rep1 |
| GSM206284 |
Chitin receptor mutant, water treatment, biological rep2 |
| GSM206285 |
Chitin receptor mutant, water treatment, biological rep3 |
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| Relations |
| Affiliated with |
GSE69995 |
| BioProject |
PRJNA101301 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE8319_RAW.tar |
27.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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