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| Status |
Public on Aug 01, 2016 |
| Title |
Expression data from human HMGN4- and HMGN2-expressing mouse embryionic fibroblasts |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
HMGN proteins, a family of non-histone nucleosome-binding proteins, maintain a dynamic balance between more and less compacted fibers via interactions with other architectural elements of chromatin.
This balance affects the ability of regulatory factors to access their target sites, which eventually modifies the expression profile of the cell.
We used microarrays to detail gene expression change in mouse fibroblasts overexpressing human HMGN4 to identify outlier genes and distinct classes of up- and down-regulated genes during this process.
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| Overall design |
Mouse embryonic fibroblasts (MEFs), MEFs, expressing hHMGN4 (MEF-N4) or hHMGN2 (MEF-N2) or vector alone (MEF-V, served as a control) were harvested from exponentially grown cell culture for RNA extraction and hybridization on Affymetrix microarrays.
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| Contributor(s) |
Kugler J, Postnikov Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 13, 2016 |
| Last update date |
Aug 02, 2016 |
| Contact name |
Yuri Postnikov |
| E-mail(s) |
PostnikY@mail.nih.gov
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| Phone |
301-496-2885
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| Organization name |
NIH
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| Street address |
37 Convent Dr
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL10740 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [probe set (exon) version] |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA325639 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE83313_RAW.tar |
57.9 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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