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Series GSE83376 Query DataSets for GSE83376
Status Public on Nov 20, 2016
Title Dynamic regulation of chromatin signatures during Drosophila embryonic glial differentiation [ChIP-Seq]
Organism Drosophila melanogaster
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Employ affinity purification approach to capture different stages of glial-specific information on gene expression, nucleosome occupancy and histone modifications (HMs) in Drosophila melanogaster embryo
 
Overall design Cell type-specific nucleosome and histone modification maps were generated from GBs and glias nuclei, which isolated from 5–7h-old gcm-Gal4,UAS-mCDGFP/cyo;UAS-3xFLAG-blrp-mCherry-RanGap and 12–14h-old repo-Gal4,UAS-3xFLAG-blrp-mCherry-RanGap/Tm3B D. melanogaster embryos by the method INTACT (Steinner et al., 2012). We determinated nucleosome occupancy in these nuclei by micrococcal nuclease sequencing (MNase-Seq). We also performed ChIP-Seq (chromatin immunoprecipitation and sequencing) for five histone modifications (H3K4me3, H3K4me1,H3K9ac, H3K27ac, and H3K27me3). Sequencing was performed on Illumina HiSeq 2000 using signle end protocol.
 
Contributor(s) Ye Y, Jiang C
Citation(s) 27634414, 31195140
Submission date Jun 15, 2016
Last update date Nov 12, 2019
Contact name Youqiong Ye
E-mail(s) youqye@gmail.com
Phone +8613661565591
Organization name Shanghai Jiao Tong University School of Medicine
Street address 280 Chongqing south road
City Shanghai
ZIP/Postal code 200025
Country China
 
Platforms (1)
GPL13304 Illumina HiSeq 2000 (Drosophila melanogaster)
Samples (12)
GSM2200374 GB_nucleosome
GSM2200375 GB_H3K4me1
GSM2200376 GB_H3K4me3
This SubSeries is part of SuperSeries:
GSE83377 Dynamic regulation of chromatin signatures during Drosophila embryonic glial differentiation
Relations
BioProject PRJNA325733
SRA SRP076594

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE83376_RAW.tar 1.4 Gb (http)(custom) TAR (of BED, BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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