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Series GSE8357 Query DataSets for GSE8357
Status Public on Dec 31, 2007
Title Functional analysis of exon 2 of p130Cas in fibroblasts derived from exon 2-specific knockout mice.
Organism Mus musculus
Experiment type Expression profiling by array
Summary p130Cas (Cas, Crk-associated substrate) is an adaptor molecule composed of an N-terminal Src homology 3 (SH3) domain, a substrate domain (SD), and a C-terminal Src binding domain (SBD). The SH3 domain of Cas has been shown to associate with various signaling molecules, including focal adhesion kinase (FAK), but its role in cellular function remains unclear. To address this issue, we established and analyzed primary fibroblasts derived from mice expressing a truncated Cas lacking the exon 2 encoding the SH3 domain (Cas exon 2∆/∆). In comparison to wild-type (Cas exon 2+/+) cells, Cas exon 2∆/∆ primary fibroblasts showed delayed migration in wound healing and reduced spreading on fibronectin (FN), which would be due to reduced complex formation of Cas exon 2∆/∆ with FAK and CrkII and also to impaired localization of Cas exon 2∆/∆ to focal adhesions on FN. In addition, to analyze downstream signaling pathway regulated by Cas exon 2, we compared gene expression profiles between Cas exon 2+/+ and Cas exon 2∆/∆ cells by a microarray analysis. Interestingly, we found that Cas exon 2-deficiency upregulated expression of CXC Chemokine Receptor-4 (CXCR4), CC Chemokine Receptor-5 (CCR5), and thrombospondin 4, which are implicated in cell motility and adhesion. These results define the role of Cas SH3-encoding exon in cell motility, FAK/Cas/CrkII complex formation, recruitment of Cas to focal adhesions, and regulation of cell adhesion-associated gene expression in primary fibroblasts.
Keywords: SH3-encoding exon, p130Cas
Overall design RNA samples extracted from Cas exon 2+/+, Cas exon 2+/∆, and Cas exon 2∆/∆ fibroblasts (12.5dpc, two embryos for each genotype) were labeled and hybridized on Affymetrix microarray. Gene expression patterns of Cas exon 2∆/∆ fibroblasts were compared with those of Cas exon 2+/+ and Cas exon 2+/∆ cells.
Contributor(s) Hiyama E, Tazaki T, Honda H
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Submission date Jul 03, 2007
Last update date Feb 11, 2019
Contact name Eiso Hiyama
Phone 81822575951
Organization name Hiroshima University
Department Natural Science Center for Basic Research and Deve
Lab LIfe Science
Street address 1-2-3, Kasumi, Minami-ku
City Hiroshima
State/province Hiroshima
ZIP/Postal code 734-8551
Country Japan
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM207200 Expression data from Cas wild primary fibroblasts1
GSM207212 Expression data from Cas wild primary fibroblast 2
GSM207216 Expression data from Cas exon 2-hetero deficient primary fibroblast 1
BioProject PRJNA101375

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Supplementary file Size Download File type/resource
GSE8357_RAW.tar 21.5 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data provided as supplementary file

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