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| Status |
Public on Sep 01, 2016 |
| Title |
HI-LNC RNA knockdown in EndoC-betaH1 cell using lentiviral ami-aRNAs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We have previously identified hundreds of human islet lncRNAs. Here we functionally characterise 12 such lncRNAs in EndoC-betaH1 cells through loss of function studies.
Our results suggest that a number of them display transcriptional phenotypes.
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| Overall design |
We have used lentiviral based ami-RNAs to knockdown 12 human islet expressed lncRNAs (HI-LNCs) in the beta cell line EndoC-betaH1. EndoC-betaH1cells were transduced with virus for 80 hrs prior to RNA-harvest followed by hybridisation to HTA-2.0 affymetrix microarrays. Each experiment was done in replicates and 5 non targetinf (NT) controls were used.
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| Contributor(s) |
Akerman I, Ravassard P, Benzara M, Sauty-Colace C, Ferrer J |
| Citation(s) |
28041957 |
| Submission date |
Jun 22, 2016 |
| Last update date |
Oct 29, 2018 |
| Contact name |
ildem akerman |
| Organization name |
University of Birmingham
|
| Department |
IMSR
|
| Lab |
Akerman Lab
|
| Street address |
BR Tower, 3rd Floor, Room 333, Edgbaston,
|
| City |
birmingham |
| ZIP/Postal code |
B15 2TT |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (84)
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| Relations |
| BioProject |
PRJNA326533 |
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