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Series GSE83674 Query DataSets for GSE83674
Status Public on Mar 31, 2019
Title Singing female songbirds: Female-specific gene networks in the song area HVC
Platform organism Taeniopygia guttata
Sample organisms Serinus canaria; Ploceus bicolor; Uraeginthus cyanocephalus
Experiment type Expression profiling by array
Summary In this data set, we compared the expression data of song nuclei HVC, visual cortex entopallium, and the molecular layer of the cerebellum dissected from females and males of 3 songbird species (S. canaria, U. cyanocephalus, P. bicolor) to identify sex-specific stimulated gene expression.
Testosterone-treated male and female canaries were also included in order to study whether testosterone effect on transcriptomes is sex-specific.
Finally, testosterone-treated female canaries were compared with a rare group of spontaneously singing female canaries to study whether testosterone stimulation would be different from natural stimulation.
 
Overall design A total of 36 Female and 30 male HVC, 35 female and 30 male entopallium, and 12 female and male cerebellum (molecular layer) from 3 songbird species (S. canaria, U. cyanocephalus, P. bicolor) were sampled. The transcriptomes were compared by 2 approches: 1) male-to-female from the same species/breeding status/testosterone treatment,
2) HVC-to-entopallium of the same group. The differential expression was analyzed using ChipInspector (Genomatix) with group-wise exhaustive method, False Discovery Rate was set to zero and the minimum significant probe coverage was set at 10.
ChipInspector carries out significance analysis on the single probe level (not provided), instead, the normalized transcript level data are included in the Sample tables.
 
Contributor(s) Ko M, Frankl-Vilches C, Bakker A, Gahr M
Citation(s) 34135731
Submission date Jun 23, 2016
Last update date Jul 01, 2021
Contact name Meng-Ching Ko
E-mail(s) mcko@orn.mpg.de
Organization name Max Planck Institute for Ornithology
Department Behavioral Neurobiology
Street address Eberhard-Gwinner-Str. 6a
City Seewiesen
State/province Bayern
ZIP/Postal code 82319
Country Germany
 
Platforms (1)
GPL17605 [MPIO-ZF1s520811] Affymetrix Zebra finch Custom Array [transcript version]
Samples (155)
GSM2212315 FW_male_ENT_3 Entopallium, biological rep1
GSM2212316 FW_male_ENT_6 Entopallium, biological rep2
GSM2212317 FW_male_ENT_7 Entopallium, biological rep3
Relations
BioProject PRJNA326680

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE83674_RAW.tar 3.9 Gb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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