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Series GSE8407 Query DataSets for GSE8407
Status Public on Aug 01, 2007
Title Elucidation of the phenotypic, functional and molecular topography of a myeloerythroid progenitor cell hierarchy
Organism Mus musculus
Experiment type Expression profiling by array
Summary The major myeloid blood cell lineages, including erythrocytes, platelets, granulocytes and macrophages, are generated from hematopoietic stem cells (HSC) by differentiation through a series of increasingly more committed progenitor cells. Precise phenotypic identification and functional characterization of such intermediate progenitors has important consequences for understanding fundamental differentiation processes and is clinically relevant since such events become dysregulated in various disease settings, including leukemia. While previous studies have suggested a hierarchy for myeloid differentiation involving a common progenitor through which all myeloid lineages are derived, several recent studies have suggested that such a developmental intermediate might not be an absolute requirement. Here, we evaluated the functional in vitro and in vivo potentials of a range of prospectively isolated myeloid precursors with differential expression of CD150, Endoglin and CD41. Our studies reveal a complex hierarchy of myeloerythroid progenitors with distinct and developmentally restricted lineage potentials. Global gene expression signatures of these cellular subsets revealed expression patterns consistent with their functional capacities, while hierarchical clustering analysis provides details on their lineage relationships. These data challenge existing models of hematopoietic differentiation, by suggesting that progenitors of the innate and adaptive immune system in the adult separate late, and to a large extent, following the divergence of megakaryocytic/erythroid potential.
Keywords: global gene expression profiling of prospectively isolated murine bone marrow progenitor subset by usage of affymetrix
 
Overall design RNA was extracted from purified adult BM subsets using RNeasy mRNA purification kit (Qiagen). Subsequent handling was performed at the SweGene Affymetrix unit at Lund University (http://www.swegene.org/microarray). Briefly, RNA (arrays in triplicate for all progenitor fractions except for pre MegE for which 5 arrays were produced) was labeled and amplified according to AffymetrixTM; Small Sample Labelling Protocol v.2, with the exception that the second round of in vitro transcription (IVT) was performed using AffymetrixTM GeneChipTM Expression 3’amplification kit. Hybridization and washing was performed according to AffymetrixTM GeneChipTM Expression analysis technical manual. Chips were scanned using an AffymetrixTM GeneChipTM Scanner 3000 and scaled to a median intensity of 100. For subsequent analysis, probe level expression values were extracted using RMA (Irizarry et al., 2003) and subsequent analyses was performed using dChip software (http://biosun1.harvard.edu/complab/dchip/) following filtering (0.5<SD/mean<1,000).
 
Contributor(s) Pronk CJ, Rossi DJ, Månsson R, Attema JL, Logi Norddahl G, Kwok Fai Chan C, Sigvardsson M, Bryder D
Citation(s) 18371379
Submission date Jul 09, 2007
Last update date Feb 11, 2019
Contact name Robert Månsson
E-mail(s) robert.mansson@ki.se
Organization name Lund University
Department Hemapotoietic stem cell biology
Street address Klinikgatan 26, BMC B12
City Lund
ZIP/Postal code 221 84
Country Sweden
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (17)
GSM207676 Adult murine BM, Lin-Sca1lowKitlowIL7r+Flk2+ 1
GSM207677 Adult murine BM, Lin-Sca1lowKitlowIL7r+Flk2+ 2
GSM207678 Adult murine BM, Lin-Sca1lowKitlowIL7r+Flk2+ 3
Relations
BioProject PRJNA101465

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8407_RAW.tar 94.9 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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