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| Status |
Public on Dec 31, 2017 |
| Title |
Early gene expression in mouse hematopoietic stem cells induced by Thrombopoietin stimulation and irradiation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Thrombopoietin (TPO) was shown to prevent irradaition-induced hematopoietic stem cells (HSCs) loss of function. A single injection of TPO to mice 45-60 min prior to irradiation is sufficient to reverse the long-lasting accumulation of persistent DNA damage. We used microarrays to analyze whether this effet can relie on TPO-induced specific transcriptional changes during this early time.
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| Overall design |
10,000 HSCs (Lin-Sca+Kit+-CD34-Flk2-) from pools of 8 to 10 C57BL/6 mice were FACS sorted and cultured in vitro for 45 min in medium containing or not TPO before irradiation. Microarrays were performed 45 min later.
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| Contributor(s) |
Porteu F, de Laval B |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 08, 2016 |
| Last update date |
Feb 21, 2018 |
| Contact name |
Florent Dumont |
| E-mail(s) |
florent.dumont@u-psud.fr
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| Organization name |
Université Paris-Saclay
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| Street address |
17 avenue des sciences
|
| City |
91400 Orsay |
| State/province |
Ile de France |
| ZIP/Postal code |
91400 |
| Country |
France |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| GSM2228885 |
HSC Pool 2 treated with TPO and IR. Biological rep.1 |
| GSM2228886 |
HSC Pool 2 treated without TPO and IR. Biological rep.1 |
| GSM2228887 |
HSC Pool 3 treated with TPO and IR. Biological rep.2 |
| GSM2228888 |
HSC Pool 3 treated without TPO and IR. Biological rep.2 |
| GSM2228889 |
HSC Pool 4 treated with TPO and IR. Biological rep.3 |
| GSM2228890 |
HSC Pool 4 treated without TPO and IR. Biological rep.3 |
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| Relations |
| BioProject |
PRJNA328265 |
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