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| Status |
Public on Jul 13, 2007 |
| Title |
Laser microdissection and microarray analysis of the hippocampus of Rasgrf1 knockout mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We used manual macrodissection or laser capture microdissection (LCM) to isolate tissue sections of the hippocampus area of Ras-GRF1 wild type and knockout mice brains, and analyzed their transcriptional patterns using commercial oligonucleotide microarrays. Comparison between the transcriptomes of macrodissected and microdissected samples showed that the LCM samples allowed detection of significantly higher numbers of differentially expressed genes, with higher statistical rates of significance. These results validate LCM as a reliable technique for in vivo genomic studies in the brain hippocampus, where contamination by surrounding areas (not expressing Ras-GRF1) increases background noise and impairs identification of differentially expressed genes. Comparison between wild type and knockout LCM hippocampus samples revealed that Ras-GRF1 elimination caused significant gene expression changes, mostly affecting signal transduction and related neural processes. The list of 36 most differentially expressed genes included loci concerned mainly with Ras/G protein signaling and cytoskeletal organization (i.e. 14-3-3γ/ζ, Kcnj6, Clasp2) or related, cross-talking pathways (i.e. jag2, decorin, strap). Consistent with the phenotypes shown by Ras-GRF1 knockout mice, many of these differentially expressed genes play functional roles in processes such as sensory development and function (i.e. Sptlc1, antiquitin, jag2) and/or neurological development/neurodegeneration processes affecting memory and learning. Indeed, potential links to neurodegenerative diseases such as Alzheimer disease (AD) or Creutzfeldt-Jacobs disease (CJD), have been reported for a number of differentially expressed genes identified in this study (Ptma, Aebp2,Clasp2, Hebp1, 14-3-3γ/ζ, Csnk1δ, etc.). These data, together with the previously described role of IRS and insulin (known Ras-GRF1 activators) in AD, warrant further investigation of a potential functional link of Ras-GRF1 to neurodegenerative processes.
Keywords: knockout analysis
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| Overall design |
6 samples of tissue sections of the hippocampus area of wild type (WT) and Rasgrf1 knockout (KO) mice brains, 3 biological replicates of each.
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| Contributor(s) |
Fernández-Medarde A, Porteros A, De Las Rivas J, Nuñez A, Fuster JJ, Santos E |
| Citation(s) |
17321057 |
| Submission date |
Jul 10, 2007 |
| Last update date |
Jan 08, 2019 |
| Contact name |
Javier De Las Rivas |
| E-mail(s) |
jrivas@usal.es
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| Phone |
34 923 294819
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| Organization name |
Cancer Research Center (CiC-IBMCC)
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| Department |
CSIC and University of Salamanca
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| Lab |
Bioinformatics and Functional Genomics
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| Street address |
Campus Miguel de Unamuno
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| City |
Salamanca |
| ZIP/Postal code |
37001 |
| Country |
Spain |
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| Platforms (1) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA101481 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE8425_RAW.tar |
55.3 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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