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| Status |
Public on Jan 18, 2008 |
| Title |
IP Staufen1 |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
In human cells, Staufen1 is double-stranded RNA-binding protein involved in several cellular functions including mRNA localization, translation and decay. We used a genome wide approach to identify and compare the mRNA targets of mammalian Staufen1. The mRNA content of Staufen1 mRNPs was identified by probing DNA microarrays with probes derived from mRNAs isolated from immunopurified Staufen-containing complexes following transfection of HEK293T cells with a Stau1-HA expressor. Our results indicate that 7% of the cellular RNAs expressed in HEK293T cells are found in Stau1-containing mRNPs. There is a predominance of mRNAs involved in cell metabolism, transport, transcription, regulation of cell processes and catalytic activity.
Keywords: Immunoprecipitation, Staufen, microarray
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| Overall design |
HEK293 cells were transiantly transfected with plasmids coding for Staufen1-HA. Cell extracts were immunoprecipitated using anti-HA antibodies and co-immunoprecipitated mRNAs were purified and used to hybridize microarrays. As control, cell extract from mock transfected cells were used.
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| Contributor(s) |
Furic L, Maher-Laporte M, DesGroseillers L |
| Citation(s) |
18094122 |
| Submission date |
Jul 11, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Luc DesGroseillers |
| E-mail(s) |
luc.desgroseillers@umontreal.ca
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| Organization name |
Université de Montréal
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| Department |
Biochimie
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| Street address |
2900 Edouard Montpetit
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| City |
Montréal |
| State/province |
Québec |
| ZIP/Postal code |
H3T1J4 |
| Country |
Canada |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA101505 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE8438_RAW.tar |
138.6 Mb |
(http)(custom) |
TAR (of CEL, CHP, EXP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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