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| Status |
Public on Dec 01, 2016 |
| Title |
The KDM5 family is required for activation of pro-proliferative cell cycle genes during adipocyte differentiation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Here we have characterized the role of KDM5 family during adipocyte differentiation of 3T3-L1 preadipocytes. Notably, our study represents the first to simultaneously knock down all the KDM5 family members thereby avoiding interference from redundancy between KDM5 subtypes. Using a combination of knockdown and genome-wide ChIP-seq and RNA-seq analyses we uncover the underlying mechanisms of the inhibition of adipogenesis as well as basic aspects of KDM5 function.
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| Overall design |
Genome-wide profiling of KDM5A binding in 3T3-L1 preadipocytes and differentiating adipocytes, and profiling of H3K4me3 and gene expression in 3T3-L1 cells depleted for KDM5 family members
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| Contributor(s) |
Brier AB, Loft A, Madsen JS, Rosengren T, Nielsen R, Schmidt SF, Liu Z, Yan Q, Gronemeyer H, Mandrup S |
| Citation(s) |
27899593 |
| Submission date |
Jul 14, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Ann-Sofie Bøgh Brier |
| E-mail(s) |
a.boegh@me.com
|
| Phone |
+4522969284
|
| Organization name |
University of Southern Denmark, Odense
|
| Department |
Department of Biochemistry and Molecular Biology
|
| Lab |
Mandrup lab
|
| Street address |
Campusvej 55
|
| City |
Odense M |
| ZIP/Postal code |
5230 |
| Country |
Denmark |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (34)
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| Relations |
| BioProject |
PRJNA329107 |
| SRA |
SRP078506 |
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