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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 23, 2016 |
| Title |
Proneurogenic ligands defined by modeling developing cortex growth factor communication networks |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The neural stem cell decision to self-renew or differentiate is tightly regulated by its microenvironment. Here, we have asked about this microenvironment, focusing on growth factors in the embryonic cortex at a time when it is largely comprised of neural precursor cells (NPCs) and newborn neurons. We show that cortical NPCs secrete factors that promote their maintenance while cortical neurons secrete factors that promote differentiation. To define factors important for these activities, we used transcriptome profiling to identify ligands produced by NPCs and neurons, cell surface mass spectrometry to identify receptors on these cells, and computational modeling to integrate these data. The resultant model predicts a complex growth factor environment with multiple autocrine and paracrine interactions. We tested this communication model, focusing on neurogenesis, and identified IFNγ, Nrtn and glial-derived neurotrophic factor (GDNF) as ligands with unexpected roles in promoting neurogenic differentiation of NPCs in vivo.
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| Overall design |
We prepared E16 cortical neuron, E13 cortical precursor and co-cultured E16 cortical neuron and E13 cortical precursor cultures from three independent biological replicates.
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| Contributor(s) |
Yuzwa SA, Yang G, Borrett M, Clarke G, Cancino GI, Zahr S, Zandstra PW, Kaplan DR, Miller FD |
| Citation(s) |
27545711 |
| Submission date |
Jul 17, 2016 |
| Last update date |
Feb 21, 2018 |
| Contact name |
Freda Miller |
| E-mail(s) |
fredam@sickkids.ca
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| Organization name |
Hospital for Sick Children
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| Street address |
686 Bay St
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| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5G 0A4 |
| Country |
Canada |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (9)
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| GSM2237812 |
Mouse Cortical Neuron, Biological Replicate 1 |
| GSM2237813 |
Mouse Cortical Neuron, Biological Replicate 2 |
| GSM2237814 |
Mouse Cortical Neuron, Biological Replicate 3 |
| GSM2237815 |
Mouse Cortical Precursor, Biological Replicate 1 |
| GSM2237816 |
Mouse Cortical Precursor, Biological Replicate 2 |
| GSM2237817 |
Mouse Cortical Precursor, Biological Replicate 3 |
| GSM2237818 |
Mouse Co-Cultured Cortical Neuron and Cortical Precursor, Biological Replicate 1 |
| GSM2237819 |
Mouse Co-Cultured Cortical Neuron and Cortical Precursor, Biological Replicate 2 |
| GSM2237820 |
Mouse Co-Cultured Cortical Neuron and Cortical Precursor, Biological Replicate 3 |
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| Relations |
| BioProject |
PRJNA329432 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE84482_RAW.tar |
86.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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