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Series GSE84522 Query DataSets for GSE84522
Status Public on Aug 10, 2017
Title Circadian expression data from muscle stem cells from adult and aged mice fed with normal diet or calorie restricted
Organism Mus musculus
Experiment type Expression profiling by array
Summary Caloric restriction (CR) enhances the function of adult stem cells and significantly extends the lifespan of many organisms including rodents. CR increases the expression of Period genes in several tissues in mice, and the circadian machinery is strongly influenced by feeding and metabolic pathways.
Importantly, Bmal1-deficient mice, or Period/Timeless-deficient Drosophila are refractory to the lifespan extension induced by CR. We therefore studied whether CR could prevent the ageing related circadian reprogramming we had observed in muscle SCs.
 
Overall design Per-1 venus C57B6/J aged and adult mice were fed either ad libitum with control diet or a 30% food reduction compared to the ad libitum group intake of calorie restricted diet. The experiment was 25 week-long and started after an adaption period of 3 weeks, where calorie restricted animals were subjected to a 10% food reduction per week. The age groups were composed of 19-29 week old and 55-69 week old mice at the start of the adaption period. Four mice were used per each of the 6 time points (ZT0, ZT4, ZT8, ZT12, ZT16, ZT20) and per diet group. For sample collection, one mouse per diet group was sacrificed per day, all belonging to one time point.
 
Contributor(s) Solanas G, Peixoto F, Perdiguero E, Ruiz-Bonilla V, Datta D, Symeonidi A, Muñoz-Cánovas P, Aznar Benitah S
Citation(s) 28802040
Submission date Jul 18, 2016
Last update date Aug 06, 2018
Contact name Aikaterini Symeonidi
E-mail(s) ksymeonidh@gmail.com
Organization name Institut for Research in Biomedicine (IRB-Barcelona)
Department Oncology
Lab Stem cells and Cancer Lab
Street address Baldiri i Reixac, 10
City Barcelona
State/province Barcelona
ZIP/Postal code 08028
Country Spain
 
Platforms (1)
GPL11180 [HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate
Samples (104)
GSM2241329 satellite cells adult animal control diet T0, biological replicate 1
GSM2241330 satellite cells adult animal control diet T0, biological replicate 2
GSM2241331 satellite cells adult animal control diet T0, biological replicate 3
This SubSeries is part of SuperSeries:
GSE84580 Adult stem cells undergo circadian reprogramming during ageing
Relations
BioProject PRJNA330309

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE84522_RAW.tar 221.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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