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| Status |
Public on Mar 06, 2017 |
| Title |
Effect of BCL11B overexpression on transcriptome of T-cell acute lymphoblastic leukemia (T-ALL) cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate the effects of BCL11B on T-cell differentiation, we performed gain of function studies in cells with a T-lineage differentiation arrest, namely T-ALL cells. Gene expression profiling by RNA-Seq demonstrated that BCL11B overexpression induced transcriptional changes consistent with T-cell differentiation as early as 72 hours after transduction, indicating a rapid regulatory effect of BCL11B on the T-lineage transcriptional program and supporting an important role for BCL11B in human T-cell differentiation.
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| Overall design |
T-ALL cells were transduced with a BCL11B-GFP expression vector (overexpressing cells) or an empty GFP vector (control cells). GFP+ cells were isolated by fluorescence activation cell sorting (FACS) at 72 hours post transduction and analyzed by RNA-Seq to determine the effect of BCL11B on the transcriptome of T-ALL cells.
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| Contributor(s) |
Parekh C |
| Citation(s) |
28232744 |
| Submission date |
Jul 21, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
chintan parekh |
| E-mail(s) |
cparekh@chla.usc.edu
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| Organization name |
Children's Hospital Los Angeles
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| Department |
Pediatrics
|
| Street address |
4650 sunset blvd, mail stop 54
|
| City |
Los Angeles |
| State/province |
California |
| ZIP/Postal code |
90027 |
| Country |
USA |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA330819 |
| SRA |
SRP079175 |
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