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| Status |
Public on Jul 23, 2016 |
| Title |
Claudin-based barrier differentiation in the colonic epithelial crypt niche involves Hopx/Klf4 and Tcf7l2/Hnf4-alpha cascades |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Analysis of the complex expression of claudin genes and transcription factors during intestinal epithelial cell differentiation across the crypt-to-surface colonic axis. Characterization of the complex expression gradients of transcription factors Hopx, Hnf4a, Klf4 and Tcfl2 and 12 claudin genes. In vitro confirmatory methods identified two pathways that stimulate claudin expression; Hopx/Klf4 activation of Cldn4, 7 and 15, and Tcf7l2/Hnf4a upregulation of Cldn23. Chromatin Immunoprecipitation confirmed a Tcf7l2/Hnf4a/Claudin23 cascade. Hnf4a conditional knockout mice fail to induce Cldn23 during colonocyte differentiation.
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| Overall design |
The distal colon tissue was obtained from 4 male, 10-12 week old, C57BL/6 (WT) mice. The Arcturus Laser Capture Microdissection (LCM) system (Applied Biosystems/Life Technologies) was used to isolate crypt-base and surface cell populations providing two samples per mouse (crypt-base and surface). This resulted in 8 total samples for microarray analysis.
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| Contributor(s) |
Capaldo CT |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 22, 2016 |
| Last update date |
Jul 25, 2016 |
| Contact name |
Loukia Lili |
| E-mail(s) |
loukialili@gmail.com
|
| Phone |
4046422896
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| Organization name |
The Institute for Next Generation Healthcare
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| Department |
Icahn School of Medicine at Mount Sinai
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| Street address |
770 Lexington Ave, 14-07 14th floor
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL6105 |
Illumina mouse-6 v1.1 expression beadchip |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA330979 |
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