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Series GSE8488 Query DataSets for GSE8488
Status Public on Jan 31, 2008
Title Inhibitor Trials
Organism Mus musculus
Experiment type Expression profiling by array
Summary Objectives: To identify similarities and differences in gene expression data in the MEK/ERK and PI3K pathways and to determine how histone modification affects these same pathways.
Goal: To identify and functionally characterize novel targets of these signaling pathways in the context of chondrocyte differentiation.
Keywords: Teatment, signaling, one-colour array, signaling pathway comparison
 
Overall design Cartilage derived from the hind limbs of CD1 mice staged at 15.5 days of embryonic development were dissected and plated in primary chondrocyte monolayer cultures. Cells were treated with MEK/ERK pathway inhibitors (SB202190 and UO126), a PI3K inhibitor (LY294002), a histone deacetylase inhibitor (Trichostatin A) and the vehicle control (DMSO) for two hours. Total RNA was isolated from these samples and hybridized to Affymetrix MOE 430 2.0 chips at the London Regional Genomics facility.
A total of 15 genechips were analyzed between 4 treatments and the vehicle (DMSO) control. Three biological replicates per treatment were executed.
 
Contributor(s) James CG, Ulici V, Beier F
Citation(s) 20111593
Submission date Jul 16, 2007
Last update date Feb 11, 2019
Contact name Claudine James
E-mail(s) claudinegj@hotmail.com, fbeier@uwo.ca
Phone (519)661-3387
Fax (519)850-2459
Organization name University of Western Ontario
Department Physiology and Pharmacology
Lab Frank Beier/ CIHR Group in Skeletal Development and Remodeling
Street address 1151 Richmond Street, Suite 2
City London
State/province Ontario
ZIP/Postal code N6A 5C1
Country Canada
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (15)
GSM209810 DMSO_1
GSM209834 DMSO_2
GSM209835 DMSO_3
Relations
BioProject PRJNA101581

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Supplementary file Size Download File type/resource
GSE8488_RAW.tar 60.3 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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