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Series GSE8498 Query DataSets for GSE8498
Status Public on Aug 04, 2007
Title The MicroRNA miR-124 Promotes Neuronal Differentiation by Triggering Brain-Specific Alternative Pre-mRNA Splicing
Organism Mus musculus
Experiment type Expression profiling by array
Summary Both microRNAs and alternative pre-mRNA splicing have been implicated in the development of the nervous system (NS), but functional interactions between these two pathways are poorly understood. We demonstrate that the neuron-specific microRNA miR-124a directly targets PTBP1/PTB/hnRNPI mRNA, which encodes a global repressor of alternative pre-mRNA splicing in non-neuronal cells. Among the targets of PTBP1 is a critical cassette exon in the pre-mRNA of PTBP2/nPTB/brPTB, an NS-enriched PTBP1 homolog. When this exon is skipped, PTBP2 mRNA is subject to nonsense-mediated decay. During neuronal differentiation, miR-124a reduces PTBP1 levels leading to the accumulation of correctly spliced PTBP2 mRNA and a dramatic increase in PTBP2 protein. These events culminate in the transition from non-NS to NS-specific alternative splicing patterns. We also present evidence that miR-124a plays a key role in the differentiation of progenitor cells to mature neurons. Thus, miR-124a promotes NS development at least in part by regulating an intricate network of NS-specific alternative splicing.
We used microarrays to detail the global programme of gene expression of CAD cells over-expressing miR-124a-2.
Keywords: treatment versus control
 
Overall design Expression data from CAD cells transfected with plasmid expressing miR-124a-2.
 
Contributor(s) Makeyev EV, Zhang J, Carrasco M, Maniatis T
Citation(s) 17679093
Submission date Jul 16, 2007
Last update date Feb 11, 2019
Contact name Jiangwen Zhang
E-mail(s) jzhang@cgr.harvard.edu
Organization name Harvard University
Department FAS Center for Systems Biology
Street address 7 Divinity Ave
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM210755 CAD_C1
GSM210756 CAD_C2
GSM210757 CAD_C3
Relations
BioProject PRJNA101601

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8498_RAW.tar 35.7 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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