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Series GSE8503 Query DataSets for GSE8503
Status Public on Jan 01, 2008
Title mRNA expression analysis of undifferentiated Dicer -/- (27H10) embryonic stem cells after miRNA transfection
Organism Mus musculus
Experiment type Expression profiling by array
Summary We have analyzed the transcript expression levels in Dicer knock-out embryonic stem (ES) cells 24 hours after transfection with either control siRNA agains Renilla luciferase or miRNA Mimics (Dharmacon) of mmu-miR-290 cluster members in order to identify primary targets of miR-290 cluster miRNAs.
Keywords: Comparison of effect of two different transfections on transcriptome of Dicer-KO ES cells
 
Overall design Cell were analysed 24h after transfections in an undifferentiated state. Triplicates of both transfections were analyzed.
 
Contributor(s) Sinkkonen L, Svoboda P, Filipowicz W
Citation(s) 18311153
Submission date Jul 17, 2007
Last update date Feb 11, 2019
Contact name Lasse Sinkkonen
E-mail(s) lasse.sinkkonen@fmi.ch
Organization name Friedrich Miescher Institute
Street address Maulbeerstrasse 66
City Basel
ZIP/Postal code 4058
Country Switzerland
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM210972 Undifferentiated Dicer -/- (27H) ES cell line transfected with siRL replicate_1
GSM210973 Undifferentiated Dicer -/- (27H) ES cell line transfected with siRL replicate_2
GSM210974 Undifferentiated Dicer -/- (27H) ES cell line transfected with siRL replicate_3
Relations
BioProject PRJNA101611

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8503_RAW.tar 20.1 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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