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| Status |
Public on Jan 01, 2008 |
| Title |
mRNA expression analysis of undifferentiated Dicer -/- (27H10) embryonic stem cells after miRNA transfection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We have analyzed the transcript expression levels in Dicer knock-out embryonic stem (ES) cells 24 hours after transfection with either control siRNA agains Renilla luciferase or miRNA Mimics (Dharmacon) of mmu-miR-290 cluster members in order to identify primary targets of miR-290 cluster miRNAs.
Keywords: Comparison of effect of two different transfections on transcriptome of Dicer-KO ES cells
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| Overall design |
Cell were analysed 24h after transfections in an undifferentiated state. Triplicates of both transfections were analyzed.
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| Contributor(s) |
Sinkkonen L, Svoboda P, Filipowicz W |
| Citation(s) |
18311153 |
| Submission date |
Jul 17, 2007 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Lasse Sinkkonen |
| E-mail(s) |
lasse.sinkkonen@fmi.ch
|
| Organization name |
Friedrich Miescher Institute
|
| Street address |
Maulbeerstrasse 66
|
| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
| |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| GSM210972 |
Undifferentiated Dicer -/- (27H) ES cell line transfected with siRL replicate_1 |
| GSM210973 |
Undifferentiated Dicer -/- (27H) ES cell line transfected with siRL replicate_2 |
| GSM210974 |
Undifferentiated Dicer -/- (27H) ES cell line transfected with siRL replicate_3 |
| GSM210975 |
Undifferentiated Dicer -/- (27H) ES cell line transfected with miR-290 cluster replicate_1 |
| GSM210976 |
Undifferentiated Dicer -/- (27H) ES cell line transfected with miR-290 cluster replicate_2 |
| GSM210977 |
Undifferentiated Dicer -/- (27H) ES cell line transfected with miR-290 cluster replicate_3 |
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| Relations |
| BioProject |
PRJNA101611 |
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