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| Status |
Public on Nov 28, 2017 |
| Title |
Control of gene expression in senescence through transcriptional read-through of convergent protein-coding genes [RNA-Seq siRNA H2A.Z] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Antisense RNAs are non-coding RNAs, which can regulate their corresponding sense RNAs and are generally produced from specific promoters. By genome-wide approaches and in depth analyses at specific loci in human cells undergoing senescence, we uncover a family of antisense RNAs produced by transcriptional read-through at convergent protein-coding genes. Importantly, these antisense RNAs, that we named STARTs, repress the expression of their corresponding sense RNAs. We found that the elongation rate of RNA pol II is limited downstream of the TTS at START loci in proliferative cells. This allows transcription termination to occur before the RNA pol II reaches the convergent genes, thus preventing antisense RNA production and interference with the expression of the convergent genes. In proliferative cells, STARTs are repressed by the histone variant H2A.Z, whose local occupancy decreases in senescence. Our results thus uncover a novel mechanism of gene expression regulation, relying on the control of the expression of read-through antisense transcripts at convergent genes and underline the functional importance of the epigenetic control of RNA pol II elongation rate at intergenic regions.
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| |
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| Overall design |
Strand-specific RNA-seq datasets in si-Ctrl and in siH2A.Z- treated WI38 hTERT RAF1-ER cells (2 replicates).
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| |
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| Contributor(s) |
Muniz L, Deb M, Aguirrebengoa M, Trouche D, Nicolas E |
| Citation(s) |
29186682 |
| Submission date |
Aug 02, 2016 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Estelle Nicolas |
| E-mail(s) |
estelle.nicolas@univ-tlse3.fr
|
| Phone |
+33 5 61 55 81 11
|
| Organization name |
INSERM
|
| Lab |
UMR5077, MCD, CBI
|
| Street address |
118 route de Narbonne
|
| City |
Toulouse |
| ZIP/Postal code |
31062 |
| Country |
France |
| |
|
| Platforms (2) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (4)
|
| GSM2781955 |
Strand-specific RNA-seq in siCtrl-treated proliferative WI38 hTERT RAF1-ER cells- Replicate #1 |
| GSM2781956 |
strand-specific RNA-seq in siH2A.Z-treated proliferative WI38 hTERT RAF1-ER cells- Replicate #1 |
| GSM2781957 |
Strand-specific RNA-seq in siCtrl-treated proliferative WI38 hTERT RAF1-ER cells- Replicate #2 |
| GSM2781958 |
Strand-specific RNA-seq in siH2A.Z-treated proliferative WI38 hTERT RAF1-ER cells- Replicate #2 |
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| This SubSeries is part of SuperSeries: |
| GSE85085 |
Control of gene expression in senescence through transcriptional read-through of convergent protein-coding genes |
|
| Relations |
| BioProject |
PRJNA336105 |
| SRA |
SRP080790 |
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