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| Status |
Public on Jan 01, 2017 |
| Title |
Gene expression profiles in the striatum of the R6/2 mouse model of Huntington's disease injected with LNA-oligonucleotides complementary to the CAG repeats (LNA-CTG) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We investigated gene expression signatures in the striatum of wild-type mice injected with a control/scrambeled LNA-oligonucleotide (LNA-SCB) and R6/2 mice injected with LNA-SCB or LNA-CTG. Five mice were used per condition. Each group of mice received two consecutive intrastriatal injections of LNA-CTG or LNA-SCB (0.05 nmols per injection, separated by 3 days) at 11 weeks of age , when motor symptoms appeared in the R6/2 mice. Injection of LNA-CTG resulted in a signifficant attenuation of the R6/2 mice motor defficits, as early as one week after administration (12 weeks of age) and persisted for at least 4 additional weeks (15 weeks of age). Overal gene expression in each group was evaluated short before motor improvement was observed (5 days post-injection), to detect gene expression changes and signalling pathway alterations underlying the benefficial effects. Gene expression was assessed with Agilent SurePrint G3 Mouse GE 8 × 60K Microarray. Target genes were validated using real-time quantitative PCR.
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| Overall design |
15 striatal tissue samples were analyzed, using Agilent technology: 5 wild-type, LNA-SCB samples; 5 R6/2, LNA-SCB samples; 5 R6/2, LNA-CTG samples. An internal control sample was included UMRR
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| Contributor(s) |
Rué L, Bañez-Coronel M, Creus-Muncunill J, Giralt A, Alcalá-Vida R, Mentxaka G, Kagerbauer B, Zomeño-Abellán MT, Aranda Z, Venturi V, Pérez-Navarro E, Estivill X, Martí E |
| Citation missing |
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| Submission date |
Aug 03, 2016 |
| Last update date |
Jul 19, 2017 |
| Contact name |
Eulalia Marti |
| E-mail(s) |
eulalia.marti@crg.eu
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| Organization name |
Centre for Genomic Regulation
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| Street address |
Dr. Aiguader 88
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| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
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| Platforms (1) |
| GPL13912 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA336269 |
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