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| Status |
Public on Jun 08, 2017 |
| Title |
Transcriptional effects of soluble CD40 ligand on human naïve B cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We performed microarray analysis to derive gene signatures down-stream of soluble CD40 ligand stimulation in human naive B cells. Naïve B cells were purified from healthy donor PBMC using negative selection beads (Miltenyi) and cultured with sCD40L at 2.5ug/ml for 6hr before microarray analysis. In the same study, cells were also harvested at day 5 post-stimulation to confirm sCD40L-induced B cell activation and proliferation. FACS analysis confirmed soluble CD40L induced up-regulation of CD86 and CD69 at 24hr. B cell proliferation was measured at day 4 post-stimulation by EdU incorporation.
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| |
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| Overall design |
Naïve B cells were purified from peripheral blood of 6 healthy donors. Naïve B cells were then stimulated with sCD40L or mock-treated, and harvested at 6hr for microarray analysis. 2-4 replicates were available per donor and condition.
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| Web link |
http://www.jimmunol.org/content/198/11/4490
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| |
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| Contributor(s) |
Walsh AM, Guo Y |
| Citation(s) |
28455435 |
| Submission date |
Aug 12, 2016 |
| Last update date |
Apr 20, 2018 |
| Contact name |
Alice Walsh |
| E-mail(s) |
awalsh17@its.jnj.com
|
| Organization name |
Janssen R&D
|
| Department |
Immunology
|
| Street address |
Welsh & McKean Roads
|
| City |
Spring House |
| State/province |
PA |
| ZIP/Postal code |
19477 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13158 |
[HT_HG-U133_Plus_PM] Affymetrix HT HG-U133+ PM Array Plate |
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| Samples (40)
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| Relations |
| BioProject |
PRJNA338769 |
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