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| Status |
Public on Aug 13, 2016 |
| Title |
An automatec microwell platform for large-scale single cell RNA-seq. |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report an automated microwell array platform for single cell RNA-seq with significantly improved performance over previous implementations. We demonstrate cell capture efficiencies of >50%, compatibility with commercially available barcoded mRNA capture beads, and parallel expression profiling from thousands of individual cells. We apply our system to comprehensively assess heterogeneity in gene expression of patient-derived glioma neurospheres and uncover subpopulations similar to those observed in human glioma tissue.
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| |
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| Overall design |
Performed single cell RNA-seq on thousands of cells from three cell lines.
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| Contributor(s) |
Yuan J, Sims PA |
| Citation(s) |
27670648 |
| Submission date |
Aug 12, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Peter Sims |
| Organization name |
Columbia University
|
| Street address |
3960 Broadway, 2nd Floor, Room 203AC
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL19415 |
Illumina NextSeq 500 (Homo sapiens; Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA338815 |
| SRA |
SRP081644 |
