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Status |
Public on Oct 05, 2017 |
Title |
RNA Pol II ChIP-seq analysis after THZ1 treatment in TL-Om1 cells |
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
ChIP-seq analysis was performed in an adult T-cell leukemia/lymphoma cell line (TL-Om1) to analyze DNA bindings of RNA polymerase II (Pol II) after treatment with the THZ1 CDK7 inhibitor.
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Overall design |
TL-Om1 cells were treated with control DMSO or THZ1 for 3 hours. The cells were crosslinked with formaldehyde for 10 min. DNA was enriched by chromatin-immunoprecipitation (ChIP) and analyzed by Illumina Hiseq 4000. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against RNA polymerase II (Abcam, ab817: 8WG16).
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Contributor(s) |
Wong RW, Ngoc PC, Leong WZ, Yam AW, Sanda T |
Citation(s) |
28978570 |
Submission date |
Aug 16, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Phuong Cao Thi Ngoc |
Organization name |
Lund University
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Street address |
BMC, B1222b, Klinikgatan 26
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City |
Lund |
ZIP/Postal code |
SE-221 84 |
Country |
Sweden |
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Platforms (1) |
GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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Samples (4)
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This SubSeries is part of SuperSeries: |
GSE85695 |
Identification of Super-enhancer-associated Cancer Genes Provides Novel Therapeutic Targets in Adult T-cell Leukemia/Lymphoma |
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Relations |
BioProject |
PRJNA339127 |
SRA |
SRP082213 |