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| Status |
Public on May 01, 2017 |
| Title |
Inhalation and oropharyngeal aspiration exposure to rod-like carbon nanotubes induces highly similar airway inflammation and biological pathways in mouse lungs |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Inhalation and low-dose aspiration elicit highly similar pulmonary inflammation which was confirmed by activation of essentially similar biological pathways and even the same genes. Present results provide evidence that oropharyngeal aspiration is valid approach for hazard assessment of nanomaterials and proper alternative method for inhalation exposure.
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| Overall design |
To compare two alternative airway exposure methods, C57BL/6 mice were exposed to rCNT by inhalation method (aerosol mass concentrations of 6.2-8.2 mg/m3; 4h/day for 4 days), or by oropharyngeal aspiration. Low-dose (10 ug/day in 50 ul of PBS ) or high-dose (40 ug/day in 50ul of PBS) aspiration exposure of rCNT was used for four days. Bronchoalveolar lavage and lung tissue samples were collected, histological sections analyzed and mRNA expression profiles were determined by Agilent microarray. Untreated mice were used as Controls for inhalation, and vehichle treated (PBS) as Controls for aspiration.
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| Contributor(s) |
Kinaret P, Ilves M, Fortino V, Rydman E, Piia K, Wolff H, Savolainen K, Greco D, Alenius H |
| Citation(s) |
28045493 |
| Submission date |
Aug 16, 2016 |
| Last update date |
Jul 19, 2017 |
| Contact name |
Dario Greco |
| E-mail(s) |
dario.greco@tuni.fi
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| Organization name |
Tampere University
|
| Department |
Faculty of Medicine and Health Technology
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| Lab |
Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
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| Street address |
Arvo ylpön Katu 34
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| City |
Tampere |
| ZIP/Postal code |
33520 |
| Country |
Finland |
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| Platforms (1) |
| GPL13912 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version) |
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| Samples (15)
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| GSM2282060 |
rCNT aspiration 4days + 24h M1_4d24h |
| GSM2282061 |
rCNT aspiration 4days + 24h M3_4d24h |
| GSM2282062 |
Vehicle (PBS) aspiration 4days + 24h C2_4d24h |
| GSM2282063 |
Vehicle (PBS) aspiration 4days + 24h C4_4d24h |
| GSM2282064 |
Untreated inhalation control 4 days + 24h UT2 |
| GSM2282065 |
Untreated inhalation control 4 days + 24h UT3 |
| GSM2282066 |
rCNT inhalation 4days + 24h M2_4h.4d.24h |
| GSM2282067 |
Untreated inhalation control 4 days + 24h UT4 |
| GSM2282068 |
Vehicle (PBS) aspiration 4days + 24h C1_4d24h |
| GSM2282069 |
Vehicle (PBS) aspiration 4days + 24h C3_4d24h |
| GSM2282070 |
rCNT aspiration 4days + 24h M2_4d24h |
| GSM2282071 |
rCNT aspiration 4days + 24h M4_4d24h |
| GSM2282072 |
vehicle (PBS) aspiration 4days + 24h UT1 |
| GSM2282073 |
rCNT inhalation 4days + 24h M1_4h.4d.24h |
| GSM2282074 |
rCNT inhalation 4days + 24h M3_4h.4d.24h |
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| Relations |
| BioProject |
PRJNA339197 |
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