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| Status |
Public on Aug 19, 2016 |
| Title |
Comprehensive Genomic Characterization of Five Canine Lymphoid Tumor Cell Lines |
| Organism |
Canis lupus familiaris |
| Experiment type |
Expression profiling by array
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| Summary |
Background Leukemia/lymphoma cell lines have been critical in the investigation of the pathogenesis and therapy of hematological malignancies. While human LL cell lines have generally been found to recapitulate the primary tumors from which they were derived, appropriate characterization including cytogenetic and transcriptional assessment is crucial for assessing their clinical predictive value. Results In the following study, five canine LL cell lines, CLBL-1, Ema, TL-1 (Nody-1), UL-1, and 3132, were characterized using extensive immunophenotyping, karyotypic analysis, oligonucleotide array comparative genomic hybridization (oaCGH), and gene expression profiling. Genome-wide DNA copy number data from the cell lines were also directly compared with 299 primary canine round cell tumors to determine whether the cell lines represent primary tumors, and, if so, what subtype each most closely resembled. Discussion Based on integrated analyses, CLBL-1 was classified as B-cell lymphoma, Ema and TL-1 as T-cell lymphoma, and UL-1 as T-cell acute lymphoblastic leukemia. 3132, originally classified as a B-cell lymphoma, was reclassified as a histiocytic sarcoma based on characteristic cytogenomic properties. In combination, these data begin to elucidate the clinical predictive value of these cell lines which will enhance the appropriate selection of in vitro models for future studies of canine hematological malignancies.
GEP using GeneChip Canine Genome 2.0 array (Affymetrix, Santa Clara, CA)
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| Overall design |
Five canine lymphoid cell lines analyzed
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| Contributor(s) |
Suter SE, Breen M |
| Citation(s) |
27639374 |
| Submission date |
Aug 16, 2016 |
| Last update date |
Nov 21, 2016 |
| Contact name |
Steven E Suter |
| E-mail(s) |
sesuter@ncsu.edu
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| Phone |
919-513-0813
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| Organization name |
NC State University
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| Department |
Department of Clinical Sciences
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| Lab |
330C
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| Street address |
1051 William Moore Drive
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| City |
Raleigh |
| State/province |
NC |
| ZIP/Postal code |
27607 |
| Country |
USA |
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| Platforms (1) |
| GPL3738 |
[Canine_2] Affymetrix Canine Genome 2.0 Array |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA339162 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE85720_RAW.tar |
19.9 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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