|
| Status |
Public on May 05, 2017 |
| Title |
LCOR regulates transcription of human mammary epithelial and breast cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Normal human mammary epithelial cell (HMLE) and breast cancer MDA-MB-231 cells are engineered to knockdown or enforce expression of variety of LCOR gene products. In addition to wild-type cDNA, functional domain deficient mutants were used to elucidate mechanism of LCOR incorporated transcriptional regulation. The transcriptome profiles were determined and compared.
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| |
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| Overall design |
Two sets of experiments: (1) LKO vector control vs LCOR-KD in two cell types to determine the outcome of LCOR deficiency. (2) LEX vector control vs. (wild-type, LSKAA mutant, △HTH mutant) LCOR to confirm the LCOR outcome (as identified by KD) and determine the functional impact of individual domain.
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| |
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| Contributor(s) |
Celià-Terrassa T, Wei Y |
| Citation(s) |
28530657 |
| Submission date |
Aug 18, 2016 |
| Last update date |
Feb 29, 2024 |
| Contact name |
Yong Wei |
| E-mail(s) |
yongwei@princeton.edu
|
| Organization name |
Princeton University
|
| Department |
Molecular Biology
|
| Lab |
Kang
|
| Street address |
LTL227 Wathington Road
|
| City |
Plainsboro |
| State/province |
NJ |
| ZIP/Postal code |
08536 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
|
| Samples (12)
|
| GSM2284657 |
MDA-MB231 cells engineered with empty pLKO1 vector (Control) |
| GSM2284658 |
MDA-MB231 cells engineered with pLKO1 driven shRNA#1 targeting LCOR (KD1) |
| GSM2284659 |
HMLE cells engineered with empty pLKO1 vector (Control) |
| GSM2284660 |
HMLE cells engineered with pLKO1 driven shRNA#1 targeting LCOR (KD1) |
| GSM2284661 |
HMLE cells engineered with empty pLEX-MCS vector, replicate 1 |
| GSM2284662 |
HMLE cells engineered with empty pLEX-MCS vector, replicate 2 |
| GSM2284663 |
HMLE cells engineered with enforce expression of wildtype LCOR, replicate 1 |
| GSM2284664 |
HMLE cells engineered with enforce expression of wildtype LCOR, replicate 2 |
| GSM2284665 |
HMLE cells engineered with enforce expression of mutant LCOR LXXLL, replicate 1 |
| GSM2284666 |
HMLE cells engineered with enforce expression of mutant LCOR LXXLL, replicate 2 |
| GSM2284667 |
HMLE cells engineered with enforce expression of mutant LCOR HTH, replicate 1 |
| GSM2284668 |
HMLE cells engineered with enforce expression of mutant LCOR HTH, replicate 2 |
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| This SubSeries is part of SuperSeries: |
| GSE85808 |
miR-199a-LCOR axis regulation of mammary gland stem cell and breast cancer stem cell transcriptional programs |
|
| Relations |
| BioProject |
PRJNA339445 |
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