|
| Status |
Public on Aug 20, 2018 |
| Title |
Epigenome Mapping in pancreatic cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Genome binding/occupancy profiling of H3K4me1 and H3K4me3 upon KMT2D depletion was performed by Illumina NextSeq500 using single‐end 75bp protocol.
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| |
|
| Overall design |
Chromatin fragments, derived from siControl and siKMT2D (using 2 different siRNAs) treated cells, were immunoprecipitated with antibodies against Anti-Histone H3 (mono-methyl K4) and Anti-Histone H3 (tri-methyl K4). After purification of DNA from cell lysate by functionalized magnetic particles, libraries for next generation sequencing were prepared and analyzed using standard procedures.
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| |
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| Contributor(s) |
Iliopoulos D, Koutsioumpa M |
| Citation(s) |
30337373 |
| Submission date |
Aug 22, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
MARINA KOUTSIOUMPA |
| E-mail(s) |
mkoutsioumpa@gmail.com
|
| Phone |
3104893978
|
| Organization name |
UCLA
|
| Department |
MEDICINE
|
| Lab |
SYSTEMS BIOMEDICINE
|
| Street address |
650 Charles E. Young Dr South, CHS 33-315
|
| City |
LOS ANGELES |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA339685 |
| SRA |
SRP082491 |
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