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| Status |
Public on Dec 31, 2016 |
| Title |
Dicer maintains the identity and function of proprioceptive sensory neurons |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
During development neurons achieve a high degree of specialization (Fishell and Heintz, 2013). Over time, while continuously recycling their components and despite transcriptional noise, their own respective properties remain intact. The transcription factors that play a large role in initially establishing neuronal identity can be required for maintaining it (Deneris and Hobert, 2014). Post-transcriptional regulation is also important to differentiation (Bian and Sun, 2011) but its role in maintaining cell identity is less established. To better understand how post-transcriptional regulation might contribute to cell identity, we examined the proprioceptive neurons in the dorsal root ganglion (DRG), a highly specialized sensory class, whose properties are well established and display clear differences when compared to other neurons in the ganglion. By conditionally ablating Dicer in mice, using a parvalbumin (Pvalb) driven cre, we impaired post-transcriptional regulation in the proprioceptive sensory neuron population. KO animals display a progressive form of ataxia at the beginning of the fourth postnatal week that is mirrored by a cell-death within the DRG. Before cell-loss, expression profiling shows a reduction of proprioceptor specific genes and an increased expression of non-proprioceptive genes normally enriched in other ganglion neurons. Furthermore, although central connections of these neurons are intact, the peripheral connections to the muscle are functionally impaired. Post-transcriptional regulation is therefore necessary to retain the transcriptional identity and support functional specialization of the proprioceptive sensory neurons.
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| Overall design |
mRNA levels were examined in the lumbar 4 dorsal root ganglion parvalbumin positive neurons with and without conditional ablation of dicer driven by parvalbumin-cre.
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| Contributor(s) |
O'Toole SM, Nelson SB |
| Citation(s) |
28003412 |
| Submission date |
Aug 24, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Sean M O'Toole |
| Organization name |
FMI
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| Department |
Neuroscience
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| Lab |
Keller Lab
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| Street address |
Maulbeerstrasse 66
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| City |
Basel |
| State/province |
Basel-Stadt |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA339998 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE86019_pvalb_dicer_KO_TPM_values.csv.gz |
336.3 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Processed data are available on Series record |
| Raw data provided as supplementary file |
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