|
| Status |
Public on Mar 03, 2018 |
| Title |
RNASeq of OMP and TRPC2 neuronal population of zebrafish olfactory sensory neurons |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNASeq was performed on mRNA obtained from OMP expressing and TRPC2 expressing olfactory sensory neurons from 48hpf zebrafish embryos. Single-cell suspensions of 48hpf olfactory epithelia were FAC-sorted to obtain RFP expressing or venus expressing cells (RFP sample1: 3500 cells, RFP sample2: 5500 cells. Venus sample1: 676 cells, venus sample2:201 cells. RNA was extracted using QIAGEN RNEasy kit and cDNA was synthesized using a custom oligo-dT primer containing T7 promoter sequence courtesy of Jim Eberwine, University of Pennsylvanina. In vitro RNA amplification was carried out through one round for OMP:RFP neurons and through two rounds for TRPC2:venus neurons as per the protocol in Morris et al, 2011. Adapter-tagged libraries were synthesized using Illumina TruSeq v2.0 and deep-sequenced on HiSeq2500 to obtain ~100million reads per sample.
|
| |
|
| Overall design |
Examination of mRNA expression profiles from 2 types of olfactory sensory neurons
|
| |
|
| Contributor(s) |
Dang P, Fisher SA, Kim J, Raper JA |
| Citation(s) |
29020985 |
| Submission date |
Aug 24, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Puneet Dang |
| E-mail(s) |
puneetd@mail.med.upenn.edu
|
| Phone |
2158982180
|
| Organization name |
University of Pennsylvania
|
| Department |
Neuroscience
|
| Lab |
Jonathan Raper
|
| Street address |
3610 Hamilton Walk
|
| City |
PHILADELPHIA |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL14875 |
Illumina HiSeq 2000 (Danio rerio) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA340009 |
| SRA |
SRP168138 |
Less...
More...