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| Status |
Public on Nov 30, 2017 |
| Title |
Effect of cardiac specific KO of p38 MAPKα on gene expression profile of the heart |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The gene expression profile of cardiomyocyte specific KO hearts was compared to equally treated control hearts after 48 hours of Angiotensin II-treatment via osmotic mini pumps. KO hearts develop during this treatment a dilative cardiomyopathy.
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| Overall design |
Gene expression of mouse hearts of conditional p38 KO mice. Mice express tamoxifen inducible cre recombinase mercremer under control of α-MHC promotor and a p38 MAPKα gene, which has loxPside-flanked exons 2 and 3. At age of 6 weeks mice were treated with 500µg hydroxytamoxifen (OH-Tx) per day for 10 days to induce the specific KO of p38 MAPKα in cardiomyocytes. Control mice express the floxed p38MAPKα gene and were treated with OH-Tx equally. For RNA isolation four hearts of control and KO mice were harvested from 3 month old male mice after 48 hours treatment with Angiotensin II (AngII) (1.5mg/kg/d). RNA was isolated from heart apex.
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| Contributor(s) |
Bottermann K, Leitner L, Pfeffer M, Nemmer J, Deenen R, Köhrer K, Stegbauer J, Gödecke A |
| Citation missing |
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| Submission date |
Aug 25, 2016 |
| Last update date |
Feb 02, 2018 |
| Contact name |
Karl Koehrer |
| E-mail(s) |
rene.deenen@hhu.de
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| Organization name |
University of Duesseldorf
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| Department |
BMFZ
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| Lab |
GTL
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| Street address |
Universitaetsstr. 1
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| City |
Duesseldorf |
| ZIP/Postal code |
40225 |
| Country |
Germany |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA340178 |
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