|
| Status |
Public on Aug 27, 2016 |
| Title |
RNA-seq study of directed sclerotome, Lon non-coding RNA PEAT, and the embryonic Dll1 lineage form E9.0-9.5 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We sequenced RNA from embryonic mouse tissue directed to form sclerotome in culture. Using Crispr/Cas9, we generated a mouse line bearing a complete deletion of the PEAT, a LncRNA adjacent tp Pax1 and highly enriched in sclerotome. We perform RNA-seq on samples from 4 stages of maturation of the Dll1 lineage.
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| |
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| Overall design |
Examination of sclerotome using RNA-seq
|
| |
|
| Contributor(s) |
Stafford DA, Harland RM |
| Citation(s) |
27986952 |
| Submission date |
Aug 26, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Richard Harland |
| E-mail(s) |
harland@berkeley.edu
|
| Phone |
(510) 643-6003
|
| Organization name |
UC Berkeley
|
| Department |
Department of Molecular and Cell Biology
|
| Street address |
571 Life Sciences Addition #3200
|
| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720-3200 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (29)
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|
| Relations |
| BioProject |
PRJNA340283 |
| SRA |
SRP083075 |
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