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| Status |
Public on Sep 01, 2016 |
| Title |
Foxo3 drives pathogenic Th1 differentiation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We showed that the transcription factor Foxo3 played a specific role in the polarization of CD4+ T cells towards pathogenic Th1 cells producing both interferon-γ (IFN-γ) and granulocyte monocyte colony stimulating factor (GM-CSF).
To understand the molecular mechanisms whereby Foxo3 controls CD4+ T cell differentiation, unbiased analysis of genes differentially expressed in Foxo3-deficient vs. Foxo3-sufficient CD4+ T cells was achieved using both resting and activated CD4+ T cells obtained following 12 or 24 hours of stimulation with anti-CD3 mAbs.
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| Overall design |
Gene expression analysis were performed on purified naive CD4+ T cells from Foxo3-/- (n=4) or WT (n=4) littremate controls either unstimulated (T0) or stimulated with 2 μg/ml of anti-CD3 mAbs for 12 (T12) or 24 (T24) hours
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| Contributor(s) |
Dejean AS, Stienne C, Michieletto MF, LIPPI Y |
| Citation(s) |
27742544 |
| Submission date |
Aug 31, 2016 |
| Last update date |
Jun 26, 2019 |
| Contact name |
Anne S Dejean |
| E-mail(s) |
anne.dejean@inserm.fr
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| Phone |
+33562744536
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| Organization name |
INSERM
|
| Department |
UMR1043
|
| Lab |
Centre de physiopathologie Toulouse Purpan
|
| Street address |
Hopital Purpan
|
| City |
Toulouse |
| State/province |
Haute-Garonne |
| ZIP/Postal code |
31024 |
| Country |
France |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA341366 |
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