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| Status |
Public on Jun 09, 2017 |
| Title |
Menin Enhances c-Myc-mediated Transcriptional Activity To Promote Cancer Progression |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
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| Summary |
MYC is a master regulator of transcription in growing cells. Menin is an enigmatic protein that displays unique ability to either suppress or promote tumorigenesis in a context dependent manner. It's interesting to ask is there any relationship between MYC and menin.Here, we used RNA-seq to study global transcriptomic expression of MYC or MEN1 knockdown HT1080 cells to investigate whether there are any correlations between MYC- and menin- regulated gene expression. Besides, we performed ChIP-seq assays for MYC and Menin binding sequences to address whether Menin and MYC share some common binding sites on chromatin.
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| Overall design |
Human fibrosarcoma HT1080 cells were transfected with MYC shRNAs, MEN1 shRNAs or non-targeting control shRNA followed by RNA extraction and sequenced on Illumina HiSeq 4000 (Homo sapiens). And also we performed MYC ChIP-seq and Menin ChIP-seq to address whether Menin and MYC share some common binding sites on chromatin.RNA Pol II ChIP-seq for NTC and shMEN1 HT1080 cell samples were used to study the effect of Menin on RNA Pol II-mediated elongation.
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| Contributor(s) |
Wu G, Shen S, Zhang H, Gao P |
| Citation(s) |
28474697 |
| Submission date |
Sep 07, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Ping Gao |
| E-mail(s) |
pgao2@ustc.edu.cn
|
| Phone |
+86-551-63607033
|
| Organization name |
School of Life Science, University of Science and Technology of China
|
| Department |
Innovation Center for Cell Biology
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| Street address |
Huangshan Road 443
|
| City |
Hefei |
| State/province |
Anhui |
| ZIP/Postal code |
230027 |
| Country |
China |
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| Platforms (2) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA342098 |
| SRA |
SRP087387 |
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