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Series GSE8658 Query DataSets for GSE8658
Status Public on Aug 20, 2007
Title PPARg regulated gene expression in human dendritic cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary In order to gain insights into how PPARg regulates different facets of dendritic cell (DC) differentiation, we sought to identify PPARg regulated genes and gene networks in monocyte-derived dendritic cells using global gene expression profiling. We employed an exogenous ligand activation approach using a selective PPARg ligand (rosiglitazone abbreviated as RSG). In addition, we have defined culture conditions in which human serum (HS) induces PPARg activation via a yet uncharacterized endogenous mechanism. We also compared the gene expression profile of developing dendritic cells obtained from patients harboring dominant negative mutations of the PPARg receptor (C114R and C131Y).
Keywords: ligand response
Overall design Monocytes were cultured for 6, 24 hours or 5 days with 500 U/ml IL-4 and 800 U/ml GM-CSF; cytokine treatment was repeated at day 3. Cells were obtained from 12 healthy individuals (6 biological replicates; the 6 and 24 hours samples were obtained from a single individual but the 5 days samples from a different one). Ligands were added at the beginning of differentiation. The 6 and 24 hours cells were treated with vehicle (DC) or 1 uM rosiglitazone (DC RSG), in the case of 5 day cultured cells 2.5 uM RSG was used. Cells were cultured in RPMI plus 10% FBS, in the case of DC4-DC6 cells were also cultured in human AB serum (DC HS). Finally we also obtained cells from patients harboring point mutations of the PPARg receptor (C114R and C131Y)
Contributor(s) Szatmari I, Nagy T, Agostini M, Chatterjee K, Nagy L
Citation(s) 17664351, 20410489, 21632707
Submission date Aug 02, 2007
Last update date Mar 25, 2019
Contact name Istvan Szatmari
Phone 36 52 416-432
Fax 36 52 314-989
Organization name University of Debrecen
Department Department of Biochemistry and Molecular Biology
Lab Nuclear Hormone Receptor
Street address Egyetem tér 1.
City Debrecen
ZIP/Postal code H-4010
Country Hungary
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (63)
GSM214734 MC1a
GSM214737 MC1b
GSM214738 MC2a
BioProject PRJNA101857

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Supplementary file Size Download File type/resource
GSE8658_RAW.tar 376.0 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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