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Series GSE8660 Query DataSets for GSE8660
Status Public on Aug 01, 2008
Title C-terminal diversity within the p53 family accounts for differences in DNA binding and transcriptional activity
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by array
Summary The p53 family is known as a family of transcription factors with functions in tumor suppression and development. Whereas the central DNA binding domain is highly conserved among the three family members p53, p63 and p73, the C-terminal domains (CTDs) are diverse and subject to alternative splicing and post-translational modification. Here we demonstrate that the CTDs strongly influence DNA binding and transcriptional activity. While p53 and the p73 isoform p73gamma have basic CTDs and form weak sequence-specific protein-DNA complexes, the major p73 isoforms alpha, beta and delta have neutral CTDs and bind DNA strongly. A basic CTD has been previously shown to enable sliding along the DNA backbone and to facilitate the search for binding sites in the complex genome. Our experiments, however, reveal that a basic CTD also reduces protein-DNA complex stability, intranuclear mobility, promoter occupancy in vivo, transgene activation and induction of cell cycle arrest or apoptosis. A basic CTD in p53 and p73gamma therefore provides both positive and negative regulatory functions presumably to enable rapid switching of protein activity in response to stress. In contrast, most p73 isoforms exhibit constitutive DNA binding activity consistent with a predominant role in developmental control.
Keywords: Adenoviros, p73 / p53
Overall design Infection of H1299 cell with different p73/p53 constructs by adenoviral system
Contributor(s) Sauer M, Bretz AC, Beinoraviciute-Kellner R, Beitzinger M, Burek CJ, Rosenwald A, Harms GS, Stiewe T
Citation(s) 18267967
Submission date Aug 02, 2007
Last update date Feb 11, 2019
Contact name Christof Burek
Phone ++49 (0)931 20147696
Organization name Universitaet Wuerzburg
Department Pathologie
Lab Rosenwald
Street address Josef-schneider-Str. 2
City Wuerzburg
ZIP/Postal code 97080
Country Germany
Platforms (2)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (11)
GSM124854 p53 family members in myogenic differentiation mock 0 h
GSM124864 p53 family members in myogenic differentiation DeltaNp73 0 h
GSM124865 p53 family members in myogenic differentiation DeltaNp73 6h
BioProject PRJNA101861

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8660_RAW.tar 10.4 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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