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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 01, 2017 |
| Title |
Lgr5-Expressing Chief Cells Drive Epithelial Regeneration and Cancer in the Oxyntic Stomach |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Adult stem cells residing within tubular glands of the corpus epithelium are believed to fuel daily tissue renewal, but their identity remains controversial. Lgr5, marks both homeostatic stem cells and “reserve” stem cells in multiple tissues. Here, we report Lgr5 expression in a subpopulation of chief cells in mouse and human corpus glands. Using a new, non-variegated Lgr5-2A-CreERT2 mouse model, we show by cell fate mapping that Lgr5-expressing chief cells do not behave as corpus stem cells under homeostatic conditions, but are recruited to function as stem cells to effect epithelial renewal following injury. In vivo ablation of the Lgr5+ cells severely impairs epithelial homeostasis in the corpus, indicating an essential role in maintaining the resident stem cell pool. We additionally define the Lgr5+ chief cells as a major cell of origin of gastric metaplasia. These findings reveal clinically relevant insights into tissue homeostasis, repair and cancer in the corpus.
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| Overall design |
Main treatment was tamoxifen versus controls, applied to three cell populations sorted based on Lgr5GFP signal intenisty (high, low, negative), 3 or 4 technical replicates from individual FACS runs
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| Contributor(s) |
Leushacke M, Tan S, Wong A, Yada S, Hajamohideen A, Tan LT, Goh J, Wong E, Denil SL, Murakami K, Barker N |
| Citation(s) |
28581476 |
| Submission date |
Sep 08, 2016 |
| Last update date |
Feb 21, 2018 |
| Contact name |
Simon Lieven Imanuel Johannes Denil |
| Organization name |
VUB/ULB
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| Department |
University Hospital Brussels
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| Lab |
BRIGHTcore
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| Street address |
Laarbeeklaan 101
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| City |
Brussels |
| ZIP/Postal code |
1090 |
| Country |
Belgium |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (21)
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| Relations |
| BioProject |
PRJNA342345 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE86603_RAW.tar |
172.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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