NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE8673 Query DataSets for GSE8673
Status Public on Jul 31, 2009
Title Molecular mechanisms of freeze injuries in commercial baker’s yeast
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by array
Summary Frozen dough baking is useful method in the modern bread-making industry. However, the fermentation activity of baker’s yeast dramatically decreased after thawing due to freeze injuries, because baker’s yeast cells contained in dough experience freeze injuries during freeze-thaw processes. Here, we performed genome-wide expression analysis to determine genetic response in baker’s yeasts under freeze-thaw condition using a DNA microarray analysis. Functional and clustering analyses in gene expression reveal that genes could be characterized by the term of freeze-thaw stress. Under short-term freeze stress (freeze treatment for 3 day), genes involved in ribosomal protein were up-regulated. Under long-term freeze stress (freeze treatment for longer than 7 day), genes involved in energy synthesis were up-regulated. In each phase, genes involved in protein damage, several stresses and trehalose and glycogen metabolism were also up-regulated. Through these freeze stress, yeast cells may improve reduced efficiency of translation and enhanced cell protection mechanism to survive under freeze stress condition. These regulations of these genes would be controlled by the cAMP-protein kinase A pathway.
Keywords: baker’s yeast, freeze-thaw stress, gene expression, freezing period
 
Overall design All experiments were done in duplicate from two independent samples.
 
Contributor(s) Murata S, Shima J
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Aug 03, 2007
Last update date Jul 01, 2016
Contact name Satomi Mizukami Murata
E-mail(s) samymm@affrc.go.jp
Phone +81-298-838-8066
Fax +81-298-838-8066
Organization name National Food Research Institute
Lab Yeast
Street address Kannondai 2-1-12
City Tsukuba
ZIP/Postal code 305-8642
Country Japan
 
Platforms (1)
GPL90 [YG_S98] Affymetrix Yeast Genome S98 Array
Samples (8)
GSM215115 Baker's yeast_freeze_0day_rep1
GSM215116 Baker's yeast_freeze_0day_rep2
GSM215117 Baker's yeast_freeze_3day_rep1
Relations
BioProject PRJNA101885

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8673_RAW.tar 27.9 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap