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| Status |
Public on Apr 18, 2019 |
| Title |
6mA-DNA-binding factor Jumu controls maternal-to-zygotic transition upstream of Zelda |
| Organism |
Drosophila melanogaster |
| Experiment type |
Methylation profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Other
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| Summary |
A long-standing question in the field of embryogenesis is how the zygotic genome is precisely activated by maternal factors, allowing normal early embryonic development. We have previously shown that N6-methyladenine (6mA) DNA modification is highly dynamic in early Drosophila embryos and forms an epigenetic mark. However, little is known about how 6mA-formed epigenetic information is decoded. Here we report that the Fox-family protein Jumu binds 6mA-marked DNA and acts as a maternal factor to regulate the maternal-to-zygotic transition. We find that zelda encoding the pioneer factor Zelda is marked by 6mA. Our genetic assays suggest that Jumu controls the proper zygotic genome activation (ZGA) in early embryos, at least in part, by regulating zelda expression. Thus, our findings not only support that the 6mA-formed epigenetic can be read by specific transcription factors, but also uncover a mechanism by which the Jumu regulates ZGA partially through Zelda in early embryos.
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| Overall design |
Different stages 6mA, mRNA profiles of jumu mutant embryos were generated by deep sequencing, using Illumina Hiseq250 and NovaSeq platform
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| Contributor(s) |
He S, Zhang G, Wang J, Gao Y, Sun R, Cao Z, Chen Z, Zheng X, Yuan J, Luo Y, Wang X, Zhang W, Zhang P, Zhao Y, He C, Tao Y, Sun Q, Chen D |
| Citation(s) |
31101825 |
| Submission date |
Sep 09, 2016 |
| Last update date |
May 29, 2019 |
| Contact name |
Shunmin He |
| E-mail(s) |
hesm@ioz.ac.cn
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| Phone |
010-64807279
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| Organization name |
Institute of Zoology, Chinese Academy of Sciences
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| Street address |
1 Beichen West Road, Chaoyang District
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| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
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| Platforms (2) |
| GPL17275 |
Illumina HiSeq 2500 (Drosophila melanogaster) |
| GPL25244 |
Illumina NovaSeq 6000 (Drosophila melanogaster) |
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| Samples (40)
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| Relations |
| BioProject |
PRJNA342625 |
| SRA |
SRP089704 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE86795_0.75h_FPKM.txt.gz |
91.1 Kb |
(ftp)(http) |
TXT |
| GSE86795_075h_jumuMut_FPKM.txt.gz |
104.7 Kb |
(ftp)(http) |
TXT |
| GSE86795_3h_DMADMut_FPKM.txt.gz |
117.6 Kb |
(ftp)(http) |
TXT |
| GSE86795_3h_FPKM.txt.gz |
95.8 Kb |
(ftp)(http) |
TXT |
| GSE86795_6h_FPKM.txt.gz |
94.9 Kb |
(ftp)(http) |
TXT |
| GSE86795_RAW.tar |
460.0 Kb |
(http)(custom) |
TAR (of BED) |
| GSE86795_jumuMutOvary_FPKM.txt.gz |
117.5 Kb |
(ftp)(http) |
TXT |
| GSE86795_jumu_mutant_FPKM.txt.gz |
209.7 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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