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Series GSE8694 Query DataSets for GSE8694
Status Public on Aug 08, 2007
Title Comparative gene expression profile analysis between native human odontoblasts and pulp tissue
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Knowledge of differential gene expression between pulp and odontoblasts might give insight to the regulation of these spatially related but functionally diverse cells. Our aim was large-scale analysis of expression profiles of native human pulp tissue and odontoblasts, and search for genes expressed only in odontoblasts.
Keywords: comparative expression analysis
 
Overall design Microarray using Affymetrix HGU133A (for pulp) and HGU133plus 2.0 array (for odontoblasts) was performed to pooled pulp and odontoblasts of native human third molars. Microarray analysis’ repeatability was estimated by comparing our pulp sample with expression profiles of two pulp samples downloaded from the GEO-database. The genes expressed only in our pulp sample or in odontoblasts were divided into categories, and the expression of selected odontoblast-specific genes of extracellular matrix (ECM) organization and biogenesis category was confirmed with RT-PCR and Western blot.
 
Contributor(s) Pääkkönen V, Vuoristo JT, Salo T, Tjäderhane L
Citation(s) 18005044
Submission date Aug 06, 2007
Last update date Mar 25, 2019
Contact name Virve Pääkkönen
E-mail(s) virve.paakkonen@oulu.fi
Organization name University of Oulu
Department Institute of Dentistry
Street address Aapistie 3
City Oulu
ZIP/Postal code 90014
Country Finland
 
Platforms (2)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (2)
GSM215142 Native odontoblast
GSM215143 Native pulp
Relations
BioProject PRJNA101917

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8694_RAW.tar 17.3 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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