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Series GSE8700 Query DataSets for GSE8700
Status Public on Aug 07, 2007
Title Expression data from epididymal fat tissues of diet induced obese rats
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Analysis of gene expression profiles of epididymal fat from DIO rats
We applied a comparative functional genomics approach to evaluate diet-induced obese (DIO) rats as an obesity model
Keywords: single time point, comparison control animal vs. diet induced obese animal
 
Overall design Gene expression profiles from DIO and lean rats were generated and compared. A private colony of male Long-Evans rats was set up at Harlan (Harlan-Sprague Dawley, Indianapolis, IN). The animals had been on either a high fat/high sucrose diet (TD95217, Harlan Takled, Madison, WI) or a regular chow (Purina 5008; Ralston-Purina, St. Louis, MO) since weaning. Rats were shipped at 14-weeks of age to our facility and maintained on a 12:12-hour light-dark photoperiod (light on 10:00 PM). They were individually housed throughout the study at ambient temperature, and had free access to diets and water. After 2 weeks acclimation to the facility, body weight of the rats and weight of food consumed in the last 7 days were measured once a week. Body fat mass was analyzed by nuclear magnetic resonance (NMR) using an Echo Medical System (Houston, TX) instrument 3 times throughout the study. Fat free mass is the difference between body mass and fat mass. All animal studies were conducted in compliance with approved institutional animal care and use protocols according to NIH guidelines (NIH publication No. 86-23, 1985). OGTT was conducted after an overnight fasting when animals were 21 weeks of age. After a baseline blood collection (time 0), animals were dosed orally with 1.5 gram/kg glucose. Subsequence blood samples were collected at 30, 60, and 120 minutes post glucose challenge for the determination of plasma glucose and insulin levels. At 25 weeks of age, blood was collected by tail bleeding after overnight fasting. Rats were killed a week later at 09:00am by rapid decapitation. Trunk blood was collected and epididymal fat tissue excised. Various circulating metabolic parameters were measured under fasted (week 25) and fed conditions (week 26). Total RNA from rat epididymal fat was isolated with RNA STAT-60 (Tel-Test) according to the manufacturer's protocol. 5 micrograms of total RNA were labeled and hybridized to Affymetrix RAE230_2 arrays according to the Affymetrix protocol.
 
Contributor(s) Li S, Chen Y
Citation(s) 18239588
Submission date Aug 06, 2007
Last update date Jul 31, 2017
Contact name Dan Li
E-mail(s) li_shuyu_dan@lilly.com
Phone 317-651-9923
Organization name Eli Lilly & Company
Street address Lilly Corporate Center
City Indianapolis
State/province IN
ZIP/Postal code 46285
Country USA
 
Platforms (1)
GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array
Samples (15)
GSM215572 Diet Induced Obese rat C1
GSM215573 Diet Induced Obese rat C2
GSM215574 Diet Induced Obese rat C3
Relations
BioProject PRJNA101929

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Supplementary file Size Download File type/resource
GSE8700_RAW.tar 38.6 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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