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Series GSE8715 Query DataSets for GSE8715
Status Public on Aug 09, 2007
Title Transcriptional Profiling of the Megabladder Mouse - A Unique Model of Bladder Dysmorphogenesis
Organism Mus musculus
Experiment type Expression profiling by array
Summary Recent studies in our lab have identified a mutant mouse model of obstructive nephropathy designated mgb for megabladder. Homozygotic mgb mice (mgb-/-) develop lower urinary tract obstruction in utero due to a lack of bladder smooth muscle differentiation. This defect is the result of a random transgene insertion into chromosome 16 followed by a translocation of this fragment into chromosome 11. In an effort to identify potential gene targets affected in mgb mice, we performed transcriptional profiling on embryonic day 15 (E15) mgb-/- bladders using both a Chromosome 11/16 Custom GeneChip Array and the Affymetrix Mouse Genome 430 2.0 GeneChip. This analysis identified no definitive mis-expressed gene targets on chromosome 11. In contrast, mgb-/- mice significantly over-expressed a cluster of gene products located on the translocated fragment of chromosome 16 including urotensin II-related peptide (Urp), which was shown to be preferentially over-expressed in developing mgb-/- bladders. Immunohistochemical studies indicated that the spatial distribution of Urp was altered in mgb-/- bladders, while biochemical studies suggested a potential role for Urp in modifying smooth muscle cell phenotype in vitro. Pathway analysis of mgb microarray data showed dysregulation of at least 60 gene products associated with the differentiation of smooth muscle. In conclusion, the results of this study indicate that the molecular pathways controlling normal smooth muscle development are severely altered in mgb-/- bladders, and provide the first evidence that Urp may play a critical role in bladder smooth muscle development.
Keywords: mgb mutant bladders
Overall design Gene expression profiling was performed on two separate study groups. First, total cellular RNA was isolated from wildtype (n=2; 1 female/1 male), mgb+/- (n=2; 1 female/1 male), and mgb-/- (n=2; 1 female/1 male) E15 whole embryos. Second, total cellular RNA was isolated from the bladders of E15 wildtype (n=7; 3 female/4 male), mgb+/- mice (n=7; 3 female/4 male), and mgb-/- mice (n=11; 7 females/4 males).
Contributor(s) Singh S, Robinson M, Ismail I, Saha M, Auer H, Kornacher K, Robinson ML, Bates CM, McHugh KM
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Submission date Aug 07, 2007
Last update date Feb 11, 2019
Contact name Kirk M McHugh
Phone 614-355-2817
Organization name Columbus Children's Hospital
Department Cell & Developmental Biology
Street address 700 Children's Drive
City Columbus
State/province OH
ZIP/Postal code 43205
Country USA
Platforms (2)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
GPL5722 Affymetrix Mus musculus 4K chromosome 11 and 16 CustomExpress Array
Samples (36)
GSM215759 mgb -/- female, whole E15 embryo (HMF_529)
GSM215760 mgb -/- male, whole E15 embryo (HMM_533)
GSM215761 mgb +/- female, whole E15 embryo (HTF_523)
BioProject PRJNA101957

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8715_430.2_CHIP_ALL_GENOTYPES.xls 16.1 Mb (ftp)(http) XLS
GSE8715_CUSTOM 11_16_BLADDER_ARRAY_ALL_GENOTYPES_FINAL_012907.xls 2.8 Mb (ftp)(http) XLS
GSE8715_CUSTOM 11_16_WHOLE_EMBRYO_ARRAY_-_FEMALE_copy.xls 1.5 Mb (ftp)(http) XLS
GSE8715_CUSTOM 11_16_WHOLE_EMBRYO_ARRAY_-_MALE_copy.xls 1.5 Mb (ftp)(http) XLS
GSE8715_RAW.tar 34.6 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data are available on Series record

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