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| Status |
Public on Nov 18, 2016 |
| Title |
Epigenome maps of time-resolved monocyte to macrophage differentiation and innate immune memory (ATAC-Seq) |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Innate immune memory is the phenomenon whereby innate immune cells such as monocytes or macrophages undergo functional reprogramming after exposure to microbial components such as LPS. We apply an integrated epigenomic approach to characterize the molecular events involved in LPS-induced tolerance in a time dependent manner. ChIP-seq, RNA-seq, WGBS and ATAC-seq data were generated. This analysis identified epigenetic programs in tolerance and trained macrophages, and the potential transcription factors involved.
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| Overall design |
Time-course in vitro culture of human monocytes. Two innate immune memory states can be induced in culture through an initial exposure of primary human monocytes to either LPS or BG for 24 hours, followed by removal of stimulus and differentiation to macrophages for an additional 5 days. Cells were collected at baseline (day 0), 1 hour, 4 hour, 24 hour and 6 days.
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| Contributor(s) |
Novakovic B, Habibi E, Wang S, Stunnenberg H |
| Citation(s) |
27863248 |
| Submission date |
Sep 22, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Hendrik G Stunnenberg |
| E-mail(s) |
H.Stunnenberg@ncmls.ru.nl
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| Phone |
+31 (0)24 3610524
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| Organization name |
Radboud University
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| Department |
Molecular Biology
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| Street address |
Geert Grooteplein 28
|
| City |
Nijmegen |
| ZIP/Postal code |
6525 GA |
| Country |
Netherlands |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (13)
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| This SubSeries is part of SuperSeries: |
| GSE85246 |
Epigenome maps of time-resolved monocyte to macrophage differentiation and innate immune memory |
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| Relations |
| BioProject |
PRJNA343921 |
| SRA |
SRP090300 |
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