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Series GSE8724 Query DataSets for GSE8724
Status Public on Aug 13, 2007
Title Gene expression analysis in the zebrafish embryos silenced by zfPGRP-SC
Organism Danio rerio
Experiment type Expression profiling by array
Summary Peptidoglycan recognition proteins (PGRPs) are a family of pattern recognition receptors (PRRs) that serve as a strong link between invertebrate and vertebrate immunity.It has been demonstrated that PGRPs in insects function as bacterial recognition molecules which trigger proteolytic or signal transduction pathways, being thus important in the antimicrobial innate immunity.
To explore the effect of zebrafish short-form PGRP (zfPGRP-SC) on proteolytic or signal transduction pathway, RNA interference (siRNA) and high-density oligonucleotide microarray methods were used to identify differentially expressed genes regulated by zfPGRP-SC.
Keywords: RNA interference
Overall design The control siRNA, zfPGRP-SC siRNA were introduced into zebrafish zygotes at the concentration of 300 ng / μl under a normal microscope.The injection volume was 1 nL per embryo. To insure the isochronous development, the embryos of one experimental and two different control samples (the group injected with the control siRNA and the uninjected group) were from the artificial fertilized embryos collected within 5 minutes. To eliminate the difference from the different parents, all embryos were randomly mixed. The microinjection was conducted within 30 minutes. Twenty-four hours later, 400 embryos for each group were collected.
Contributor(s) Chang M, Nie P
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Submission date Aug 08, 2007
Last update date Jan 25, 2018
Contact name M X Chang
Organization name Institute of Hydrobiology, Chinese Academy of Sciences
Lab Laboratory of Fish Diseases
Street address Luojiaashan
City Wuhan
ZIP/Postal code 430072
Country China
Platforms (1)
GPL1319 [Zebrafish] Affymetrix Zebrafish Genome Array
Samples (3)
GSM216437 Zebrafish-C
GSM216438 Zebrafish-control siRNA
GSM216439 Zebrafish-PGRP-SC
BioProject PRJNA101975

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Supplementary file Size Download File type/resource
GSE8724_RAW.tar 7.1 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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