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Series GSE87292 Query DataSets for GSE87292
Status Public on Sep 05, 2017
Title Transcriptomic analysis of lung tissue from cigarette smoke induced emphysema murine models and human COPD show shared and distinct pathways
Organism Mus musculus
Experiment type Expression profiling by array
Summary Although cigarette smoke (CS) is the primary risk factor for COPD, the underlying molecular mechanisms for the significant variability in developing COPD in response to CS are incompletely understood. We performed lung gene expression profiling of two different wild-type murine strains (C57BL/6J, NZW/LacJ) and two genetic models with mutations in COPD GWAS genes (HHIP, FAM13A) after 6 months of chronic CS exposure and compared the results to human COPD lung tissues. We identified gene response patterns that correlate with severity of emphysema in mouse and human lungs. Xenobiotic metabolism and Nrf2-mediated oxidative stress response were commonly regulated molecular response patterns across C57BL/6J, Hhip +/- and Fam13a -/- murine models upon chronic CS exposure and human COPD subjects. The CS resistant Fam13a -/- mouse and NZW/LacJ strain revealed an opposite pattern of gene expression response, suggesting distinct molecular pathways for resistance against emphysema. There were few genes commonly modulated between mouse and humans. Our study suggests gene expression responses to CS may be largely species and model dependent, yet shared pathways could provide biologically significant insights underlying individual susceptibility to CS
 
Overall design Total RNA was obtained from the lung tissue of C57BL/6J wild type, Hhip+/- , Fam13a -/- and NZW/LacJ exposed to cigarette smoke (CS) or filtered air (air) for 6 months and gene expression profiling was performed on murine lungs.
 
Contributor(s) Yun JH, Morrow J, Owen C, Qiu W, Glass K, Lao T, Jiang Z, Perrella M, Silverman EK, Zhou X, Hersh CP
Citation(s) 28248572
Submission date Sep 23, 2016
Last update date Jun 14, 2018
Contact name Jeong H Yun
E-mail(s) jeong.yun@channing.harvard.edu
Organization name BRIGHAM AND WOMEN'S HOSPITAL
Street address 181 Longwood Ave
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platforms (1)
GPL6885 Illumina MouseRef-8 v2.0 expression beadchip
Samples (109)
GSM2326982 C57Air [Sample001]
GSM2326983 C57Air [Sample002]
GSM2326984 C57Smoke [Sample003]
Relations
BioProject PRJNA344029

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE87292_RAW.tar 3.1 Mb (http)(custom) TAR
GSE87292_non-normalized.txt.gz 9.9 Mb (ftp)(http) TXT
Processed data included within Sample table
Raw data are available on Series record

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