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| Status |
Public on Aug 10, 2007 |
| Title |
Folate deficiency enhances arsenic effects on ODC mouse skin gene expression |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We demonstrate that expression of key markers of keratinocyte differentiation is suppressed by exposure to sodium arsenite. Folate deficiency exacerbates this effect. In addition, cancer-related cell movement genes, and growth and proliferation genes are altered. Several redox-sensitive transcription factors are implicated in mediating these gene expression changes due to arsenic treatment and folate deficiency.
Keywords: gene expression/microarray
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| Overall design |
K6/ODC mice were maintained on either a folate deficient or folate sufficient diet and exposed to 0, 1, or 10 ppm sodium arsenite in the drinking water for 30 days. Total RNA was isolated from skin samples and gene expression analyzed using Affymetrix Mouse 430 2.0 GeneChips. Data from 24 samples, with four mice in each of the six treatment groups, were analyzed.
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| Contributor(s) |
Nelson GM, Ahlborn GJ, Delker DA, Kitchin KT, O'Brien T, Chen Y, Kohan MJ, Roop BC, Ward WO, Allen JW |
| Citation(s) |
17928125 |
| Submission date |
Aug 09, 2007 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Gail M Nelson |
| E-mail(s) |
nelson.gail@epa.gov
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| Phone |
919-541-3095
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| Organization name |
U.S. Environmental Protection Agency
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| Street address |
109 TW Alexander Dr.
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| City |
Research Triangle Park |
| State/province |
NC |
| ZIP/Postal code |
27711 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA101993 |
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