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Series GSE87379 Query DataSets for GSE87379
Status Public on May 01, 2017
Title Comprehensive functional screening of miRNAs involved in human fat cell insulin sensitivity
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary The aim of this study was to detect and functionally investigate miRNAs linked to insulin sensitivity in human subcutaneous white adipose tissue (scWAT). Subjects were selected based on the insulin-stimulated lipogenesis response of subcutaneous adipocytes.
Eleven miRNAs displayed differential expression between OIR and OIS states. Overexpression of miR-143-3p and miR-652-3p increased insulin-stimulated lipogenesis in human in vitro-differentiated adipocytes. MicroRNAs-143-3p and -652-3p are linked to scWAT insulin resistance independently of obesity.
 
Overall design Global miRNA profiling by array was performed in abdominal scWAT of obese insulin resistance (OIR), obese insulin sensitive (OIS) and lean women. Signicance analysis of microarrays (SAM) was use to compare the difference in gene expression between groups.MicroRNAs demonstrating differential expression between OIR and OIS women were overexpressed in human in vitro-differentiated adipocytes followed by assessment of lipogenesis and identification of miRNA targets by measuring mRNA/protein expression and 3’UTR analysis.
 
Contributor(s) Dahlman I
Citation(s) 28270439
Submission date Sep 27, 2016
Last update date Jul 31, 2017
Contact name Ingrid Dahlman
E-mail(s) ingrid.dahlman@ki.se
Organization name Karolinska
Street address Karolinska Huddinge
City Stockholm
ZIP/Postal code 14186
Country Sweden
 
Platforms (1)
GPL8786 [miRNA-1] Affymetrix Multispecies miRNA-1 Array
Samples (48)
GSM2329944 obese insulin resistant lipogenesis ID01_751
GSM2329945 lean control ID02_884
GSM2329946 lean control ID03_1141
Relations
BioProject PRJNA344564

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE87379_RAW.tar 7.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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