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Series GSE8753 Query DataSets for GSE8753
Status Public on Dec 01, 2007
Title Sequential responses to high-fat feeding in an obese mouse model
Organism Mus musculus
Experiment type Expression profiling by array
Summary We examined the effects of high-fat diet on feeding behaviour, body weight regulation and common biomarkers associated with weight gain in the C57BL/6J mice over a period of 10 weeks, making measurements at weeks 2, 4 and 10. We examined the transcriptomic profile of hepatic genes involved in the major lipid metabolic pathways, validating the key genes with quantitative real-time reverse-transcription PCR (qRT-PCR) and their gene products with western blots.
Keywords: Lipogenesis, Hyperphagia, Fat Oxidation, Energy Regulation, Body Weight
Overall design C57BL/6J mice were randomly assigned to consume either the control (C) or HFC diet. Body weights and food intake were measured weekly and other measurements at weeks 2, 4 and 10. Microarrays were used to screen the transcriptional response of the livers at the 3 time-points. Genes, encoding enzymes regulating key steps of lipid metabolism, were then selected from the microarray data for validation by qRT-PCR and their protein expression by western blot assays.
Contributor(s) Chan M, Zhao Y, Heng C
Citation(s) 18292748
Submission date Aug 12, 2007
Last update date Feb 11, 2019
Contact name Mei Yen Chan
Organization name Yong Loo Lin School of Medicine, National University of Singapore
Department Pediatrics
Lab Cardiovascular Genetics
Street address 5 Lower Kent Ridge Road
City Singapore
State/province Singapore
ZIP/Postal code 119074
Country Singapore
Platforms (2)
GPL339 [MOE430A] Affymetrix Mouse Expression 430A Array
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM217323 Liver_HF_week2
GSM217342 Liver_HF_week4
GSM217344 Liver_HF_week10
BioProject PRJNA102023

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Supplementary file Size Download File type/resource
GSE8753_RAW.tar 20.1 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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