NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE8753 Query DataSets for GSE8753
Status Public on Dec 01, 2007
Title Sequential responses to high-fat feeding in an obese mouse model
Organism Mus musculus
Experiment type Expression profiling by array
Summary We examined the effects of high-fat diet on feeding behaviour, body weight regulation and common biomarkers associated with weight gain in the C57BL/6J mice over a period of 10 weeks, making measurements at weeks 2, 4 and 10. We examined the transcriptomic profile of hepatic genes involved in the major lipid metabolic pathways, validating the key genes with quantitative real-time reverse-transcription PCR (qRT-PCR) and their gene products with western blots.
Keywords: Lipogenesis, Hyperphagia, Fat Oxidation, Energy Regulation, Body Weight
 
Overall design C57BL/6J mice were randomly assigned to consume either the control (C) or HFC diet. Body weights and food intake were measured weekly and other measurements at weeks 2, 4 and 10. Microarrays were used to screen the transcriptional response of the livers at the 3 time-points. Genes, encoding enzymes regulating key steps of lipid metabolism, were then selected from the microarray data for validation by qRT-PCR and their protein expression by western blot assays.
 
Contributor(s) Chan M, Zhao Y, Heng C
Citation(s) 18292748
Submission date Aug 12, 2007
Last update date Feb 11, 2019
Contact name Mei Yen Chan
E-mail(s) m_y_chan3@hotmail.com
Organization name Yong Loo Lin School of Medicine, National University of Singapore
Department Pediatrics
Lab Cardiovascular Genetics
Street address 5 Lower Kent Ridge Road
City Singapore
State/province Singapore
ZIP/Postal code 119074
Country Singapore
 
Platforms (2)
GPL339 [MOE430A] Affymetrix Mouse Expression 430A Array
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM217323 Liver_HF_week2
GSM217342 Liver_HF_week4
GSM217344 Liver_HF_week10
Relations
BioProject PRJNA102023

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8753_RAW.tar 20.1 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap