 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 01, 2007 |
| Title |
Sequential responses to high-fat feeding in an obese mouse model |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We examined the effects of high-fat diet on feeding behaviour, body weight regulation and common biomarkers associated with weight gain in the C57BL/6J mice over a period of 10 weeks, making measurements at weeks 2, 4 and 10. We examined the transcriptomic profile of hepatic genes involved in the major lipid metabolic pathways, validating the key genes with quantitative real-time reverse-transcription PCR (qRT-PCR) and their gene products with western blots.
Keywords: Lipogenesis, Hyperphagia, Fat Oxidation, Energy Regulation, Body Weight
|
| |
|
| Overall design |
C57BL/6J mice were randomly assigned to consume either the control (C) or HFC diet. Body weights and food intake were measured weekly and other measurements at weeks 2, 4 and 10. Microarrays were used to screen the transcriptional response of the livers at the 3 time-points. Genes, encoding enzymes regulating key steps of lipid metabolism, were then selected from the microarray data for validation by qRT-PCR and their protein expression by western blot assays.
|
| |
|
| Contributor(s) |
Chan M, Zhao Y, Heng C |
| Citation(s) |
18292748 |
| Submission date |
Aug 12, 2007 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Mei Yen Chan |
| E-mail(s) |
m_y_chan3@hotmail.com
|
| Organization name |
Yong Loo Lin School of Medicine, National University of Singapore
|
| Department |
Pediatrics
|
| Lab |
Cardiovascular Genetics
|
| Street address |
5 Lower Kent Ridge Road
|
| City |
Singapore |
| State/province |
Singapore |
| ZIP/Postal code |
119074 |
| Country |
Singapore |
| |
|
| Platforms (2) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA102023 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE8753_RAW.tar |
20.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...