 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 31, 2017 |
| Title |
Expression data from transdetermined Drosophila eye antennal imaginal disc by winged eye overexpression. |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
|
| Summary |
Drosophila imaginal disc cells exhibit a remarkable ability to switch cell fates under various perturbations, a phenomenon known as transdetermination (TD). The winged eye (wge) gene induces eye-to-wing TD by its overexpression in eye imaginal discs using eye specific Gal4 driver (eyeless-Gal4). Gene network controlling this process, however, is largely unclear. Additionally, we identified that heterochromatin-related histone methyltransferase Su(var)3-9 is essential for wge-mediated TD.
We used microarray to detail the global gene network underlying wge-mediated eye-to-wing TD, and the involvement of Su(var)3-9 in the gene network.
|
| |
|
| Overall design |
Third instar larval eye imaginal discs were dissected for RNA extraction and hybridization on Affymetrix microarray. Flies from each genotype were used (I: eyeless-Gal4, II: UAS-wge, III: ey-Gal4; UAS-wge (ey > wge), IV: ey-Gal4 UAS-wge; Su(var)3-91/Su(var)3-92). Three biologic replicates were obtained for each.
|
| |
|
| Contributor(s) |
Masuko K, Kurata S |
| Citation(s) |
29298422 |
| Submission date |
Oct 05, 2016 |
| Last update date |
May 04, 2018 |
| Contact name |
Keita Masuko |
| E-mail(s) |
ke.masuko@gmail.com
|
| Organization name |
Tohoku University
|
| Department |
Pharmaceutical Science
|
| Street address |
Aoba 6-3, Aramaki, Aoba-ku,
|
| City |
Sendai |
| ZIP/Postal code |
980-8578 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
|
| Samples (12)
|
| GSM2335653 |
eye disc of UAS-wge, biologic rep 1 |
| GSM2335654 |
eye disc of UAS-wge, biologic rep 2 |
| GSM2335655 |
eye disc of UAS-wge, biologic rep 3 |
| GSM2335656 |
eye disc of ey > wge, biologic rep 1 |
| GSM2335657 |
eye disc of ey > wge, biologic rep 2 |
| GSM2335658 |
eye disc of ey > wge, biologic rep 3 |
| GSM2335659 |
eye disc of ey > wge; Su(var)3-9[1]/[2], biologic rep 1 |
| GSM2335660 |
eye disc of ey > wge; Su(var)3-9[1]/[2], biologic rep 2 |
| GSM2335661 |
eye disc of ey > wge; Su(var)3-9[1]/[2], biologic rep 3 |
|
| Relations |
| BioProject |
PRJNA345425 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE87622_RAW.tar |
19.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...